4L6Q

ROCK2 in complex with benzoxaborole

4L6Q の概要

• エントリーDOI: 10.2210/pdb4l6q/pdb
• 分子名称: Rho-associated protein kinase 2, 6-[4-(aminomethyl)-2-fluorophenoxy]-2,1-benzoxaborol-1(3H)-ol (3 entities in total)
• 機能のキーワード: transferase-transferase inhibitor complex, transferase/transferase inhibitor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: O75116
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 92346.38

構造登録者

Rock, F.,Jarnagin, K. (登録日: 2013-06-12, 公開日: 2013-10-02, 最終更新日: 2024-02-28)

主引用文献

Akama, T.,Dong, C.,Virtucio, C.,Sullivan, D.,Zhou, Y.,Zhang, Y.K.,Rock, F.,Freund, Y.,Liu, L.,Bu, W.,Wu, A.,Fan, X.Q.,Jarnagin, K.
Linking phenotype to kinase: identification of a novel benzoxaborole hinge-binding motif for kinase inhibition and development of high-potency rho kinase inhibitors.
J.Pharmacol.Exp.Ther., 347:615-625, 2013

PubMed Abstract: Benzoxaboroles are a novel class of drug-like compounds that have been rich sources of novel inhibitors for various enzymes and of new drugs. While examining benzoxaborole activity in phenotypic screens, our attention was attracted by the (aminomethylphenoxy)benzoxaborole family, which potently inhibited Toll-like receptor-stimulated cytokine secretion from leukocytes. After considering their structure-activity relationships and the central role of kinases in leukocyte biology, we performed a kinome-wide screen to investigate the members of the (aminomethylphenoxy)benzoxaborole family. This technique identified Rho-activated kinase (ROCK) as a target. We showed competitive behavior, with respect to ATP, and then determined the ROCK2-drug cocrystal structure. The drug occupies the ATP site in which the oxaborole moiety provides hydrogen bond donors and acceptors to the hinge, and the aminomethyl group interacts with the magnesium/ATP-interacting aspartic acid common to protein kinases. The series exhibits excellent selectivity against most of the kinome, with greater than 15-fold selectivity against the next best member of the AGC protein kinase subfamily. Medicinal chemistry efforts with structure-based design resulted in a compound with a Ki of 170 nM. Cellular studies revealed strong enzyme inhibition rank correlation with suppression of intracellular phosphorylation of a ROCK substrate. The biochemical potencies of these compounds also translated to functional activity, causing smooth muscle relaxation in rat aorta and guinea pig trachea. The series exhibited oral availability and one member reduced rat blood pressure, consistent with ROCK's role in smooth muscle contraction. Thus, the benzoxaborole moiety represents a novel hinge-binding kinase scaffold that may have potential for therapeutic use.

PubMed: 24049062
DOI: 10.1124/jpet.113.207662

実験手法

X-RAY DIFFRACTION (2.79 Å)