4L69

Rv2372c of Mycobacterium tuberculosis is RsmE like methyltransferse

4L69 の概要

• エントリーDOI: 10.2210/pdb4l69/pdb
• 分子名称: Ribosomal RNA small subunit methyltransferase E (2 entities in total)
• 機能のキーワード: methyltransferase, transferase
• 由来する生物種: Mycobacterium tuberculosis
• 細胞内の位置: Cytoplasm (By similarity): P67202
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27773.87

構造登録者

Kumar, A.,Taneja, B. (登録日: 2013-06-12, 公開日: 2014-03-19, 最終更新日: 2023-11-08)

主引用文献

Kumar, A.,Kumar, S.,Taneja, B.
The structure of Rv2372c identifies an RsmE-like methyltransferase from Mycobacterium tuberculosis
Acta Crystallogr.,Sect.D, 70:821-832, 2014

PubMed Abstract: U1498 of 16S rRNA plays an important role in translation fidelity as well as in antibiotic response. U1498 is present in a methylated form in the decoding centre of the ribosome. In this study, Rv2372c from Mycobacterium tuberculosis has been identified as an RsmE-like methyltransferase which specifically methylates U1498 of 16S rRNA at the N3 position and can complement RsmE-deleted Escherichia coli. The crystal structure of Rv2372c has been determined, and reveals that the protein belongs to a distinct class in the SPOUT superfamily and exists as a dimer. The deletion of critical residues at the C-terminus of Rv2372c leads to an inability of the protein to form stable dimers and to abolition of the methyltransferase activity. A ternary model of Rv2372c with its cofactor S-adenosylmethionine (SAM) and the 16S rRNA fragment (1487)16S rRNA(1510) helps to identify binding pockets for SAM (in the deep trefoil knot) and substrate RNA (at the dimer interface) and suggests an S(N)2 mechanism for the methylation of N3 of U1498 in 16S rRNA.

PubMed: 24598751
DOI: 10.1107/S1399004713033555

実験手法

X-RAY DIFFRACTION (2.9 Å)