4L37

SP2-SP3 - a complex of two storage proteins from Bombyx mori hemolymph

4L37 の概要

• エントリーDOI: 10.2210/pdb4l37/pdb
• 関連するPDBエントリー: 3GWJ
• 分子名称: Silkworm storage protein, Arylphorin, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: arylphorin, hemocyanin-like fold, storage proteins, bmsp2, bmsp3, hemolymph, protein binding
• 由来する生物種: Bombyx mori (silk moth,silkworm); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 165635.04

構造登録者

Pietrzyk, A.J.,Bujacz, A.,Mueller-Dieckmann, J.,Jaskolski, M.,Bujacz, G. (登録日: 2013-06-05, 公開日: 2013-12-04, 最終更新日: 2024-11-20)

主引用文献

Pietrzyk, A.J.,Bujacz, A.,Mueller-Dieckmann, J.,ochynska, M.,Jaskolski, M.,Bujacz, G.
Crystallographic identification of an unexpected protein complex in silkworm haemolymph.
Acta Crystallogr.,Sect.D, 69:2353-2364, 2013

PubMed Abstract: The first crystal structure of a complex formed by two storage proteins, SP2 and SP3, isolated from their natural source, mulberry silkworm (Bombyx mori L.) haemolymph, has been determined. The structure was solved by molecular replacement using arylphorin, a protein rich in aromatic amino-acid residues, from oak silkworm as the initial model. The quality of the electron-density maps obtained from the X-ray diffraction experiment allowed the authors to detect that the investigated crystal structure was composed of two different arylphorins: SP2 and SP3. This discovery was confirmed by N-terminal sequencing. SP2 has been extensively studied previously, whereas only a few reports on SP3 are available. However, to date no structural studies have been reported for these proteins. These studies revealed that SP2 and SP3 exist in the silkworm body as a heterohexamer formed by one SP2 trimer and one SP3 trimer. The overall fold, consisting of three haemocyanin-like subdomains, of SP2 and SP3 is similar. Both proteins contain a conserved N-glycosylation motif in their structures.

PubMed: 24311577
DOI: 10.1107/S0907444913021823

実験手法

X-RAY DIFFRACTION (2.9 Å)