4L1E

Crystal structure of C-Phycocyanin from Leptolyngbya sp. N62DM

4L1E の概要

• エントリーDOI: 10.2210/pdb4l1e/pdb
• 関連するPDBエントリー: 1GH0 2UUM 3KVS
• 分子名称: Phycocyanin alpha chain, Phycocyanin beta chain, PHYCOCYANOBILIN, ... (5 entities in total)
• 機能のキーワード: alpha-beta dimer, light harvesting protein, thylakoid membrane, photosynthesis
• 由来する生物種: Leptolyngbya sp. N62DM; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 224617.76

構造登録者

Singh, N.K.,Raj, I.,Gourinath, S.,Madamwar, D. (登録日: 2013-06-03, 公開日: 2014-05-21, 最終更新日: 2023-09-20)

主引用文献

Singh, N.K.,Hasan, S.S.,Kumar, J.,Raj, I.,Pathan, A.A.,Parmar, A.,Shakil, S.,Gourinath, S.,Madamwar, D.
Crystal Structure and Interaction of Phycocyanin with beta-Secretase: A Putative Therapy for Alzheimer's Disease.
CNS Neurol Disord Drug Targets, 13:691-698, 2014

PubMed Abstract: Alzheimer's disease (AD) represents a neurological disorder, which is caused by enzymatic degradation of an amyloid precursor protein into short peptide fragments that undergo association to form insoluble plaques. Preliminary studies suggest that cyanobacterial extracts, especially the light-harvesting protein phycocyanin, may provide a means to control the progression of the disease. However, the molecular mechanism of disease control remains elusive. In the present study, intact hexameric phycocyanin was isolated and crystallized from the cyanobacterium Leptolyngbya sp. N62DM, and the structure was solved to a resolution of 2.6 A. Molecular docking studies show that the phycocyanin αβ-dimer interacts with the enzyme β-secretase, which catalyzes the proteolysis of the amyloid precursor protein to form plaques. The molecular docking studies suggest that the interaction between phycocyanin and β-secretase is energetically more favorable than previously reported inhibitor-β-secretase interactions. Transgenic Caenorhabditis elegans worms, with a genotype to serve as an AD-model, were significantly protected by phycocyanin. Therefore, the present study provides a novel structure-based molecular mechanism of phycocyanin-mediated therapy against AD.

PubMed: 24576002

実験手法

X-RAY DIFFRACTION (2.61 Å)