4L0A

X-ray structure of an all LNA quadruplex

4L0A の概要

• エントリーDOI: 10.2210/pdb4l0a/pdb
• 分子名称: DNA/RNA (5'-R(*(TLN)P*(LCG)P*(LCG)P*(LCG)P*(TLN))-3'), POTASSIUM ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: parallel g-quadruplex, locked nucleic acid (lna), dna, rna
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 14039.65

構造登録者

Russo Krauss, I.,Parkinson, G.,Merlino, A.,Mazzarella, L.,Sica, F. (登録日: 2013-05-31, 公開日: 2014-03-05, 最終更新日: 2023-09-20)

主引用文献

Russo Krauss, I.,Parkinson, G.N.,Merlino, A.,Mattia, C.A.,Randazzo, A.,Novellino, E.,Mazzarella, L.,Sica, F.
A regular thymine tetrad and a peculiar supramolecular assembly in the first crystal structure of an all-LNA G-quadruplex.
Acta Crystallogr.,Sect.D, 70:362-370, 2014

PubMed Abstract: Locked nucleic acids (LNAs) are formed by bicyclic ribonucleotides where the O2' and C4' atoms are linked through a methylene bridge and the sugar is blocked in a 3'-endo conformation. They represent a promising tool for therapeutic and diagnostic applications and are characterized by higher thermal stability and nuclease resistance with respect to their natural counterparts. However, structural descriptions of LNA-containing quadruplexes are rather limited, since few NMR models have been reported in the literature. Here, the first crystallographically derived model of an all-LNA-substituted quadruplex-forming sequence 5'-TGGGT-3' is presented refined at 1.7 Å resolution. This high-resolution crystallographic analysis reveals a regular parallel G-quadruplex arrangement terminating in a well defined thymine tetrad at the 3'-end. The detailed picture of the hydration pattern reveals LNA-specific features in the solvent distribution. Interestingly, two closely packed quadruplexes are present in the asymmetric unit. They face one another with their 3'-ends giving rise to a compact higher-order structure. This new assembly suggests a possible way in which sequential quadruplexes can be disposed in the crowded cell environment. Furthermore, as the formation of ordered structures by molecular self-assembly is an effective strategy to obtain nanostructures, this study could open the way to the design of a new class of LNA-based building blocks for nanotechnology.

PubMed: 24531470
DOI: 10.1107/S1399004713028095

実験手法

X-RAY DIFFRACTION (1.7 Å)