4KZX

Rabbit 40S ribosomal subunit in complex with eIF1.

Summary for 4KZX

• Entry DOI: 10.2210/pdb4kzx/pdb
• Descriptor: 40S ribosomal protein SA, 40S Ribosomal protein S9, 40S ribosomal protein S10, ... (35 entities in total)
• Functional Keywords: ribosome, translation initiation, preinitiation complex
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm: G1U2Z9
• Total number of polymer chains: 35
• Total formula weight: 1238645.85

Authors

Lomakin, I.B.,Steitz, T.A. (deposition date: 2013-05-30, release date: 2013-07-24, Last modification date: 2023-09-20)

Primary citation

Lomakin, I.B.,Steitz, T.A.
The initiation of mammalian protein synthesis and mRNA scanning mechanism.
Nature, 500:307-311, 2013

PubMed Abstract: During translation initiation in eukaryotes, the small ribosomal subunit binds messenger RNA at the 5' end and scans in the 5' to 3' direction to locate the initiation codon, form the 80S initiation complex and start protein synthesis. This simple, yet intricate, process is guided by multiple initiation factors. Here we determine the structures of three complexes of the small ribosomal subunit that represent distinct steps in mammalian translation initiation. These structures reveal the locations of eIF1, eIF1A, mRNA and initiator transfer RNA bound to the small ribosomal subunit and provide insights into the details of translation initiation specific to eukaryotes. Conformational changes associated with the captured functional states reveal the dynamics of the interactions in the P site of the ribosome. These results have functional implications for the mechanism of mRNA scanning.

PubMed: 23873042
DOI: 10.1038/nature12355

Experimental method

X-RAY DIFFRACTION (7.809 Å)