4KUL

Crystal structure of N-terminal acetylated yeast Sir3 BAH domain V83P mutant

4KUL の概要

• エントリーDOI: 10.2210/pdb4kul/pdb
• 関連するPDBエントリー: 4KUD 4KUI
• 分子名称: Regulatory protein SIR3 (2 entities in total)
• 機能のキーワード: bah domain, silencing, nucleus, transcription
• 由来する生物種: Saccharomyces cerevisiae (yeast)
• 細胞内の位置: Nucleus: P06701
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26772.28

構造登録者

Yang, D.,Fang, Q.,Wang, M.,Ren, R.,Wang, H.,He, M.,Sun, Y.,Yang, N.,Xu, R.M. (登録日: 2013-05-22, 公開日: 2013-08-07, 最終更新日: 2023-11-08)

主引用文献

Yang, D.,Fang, Q.,Wang, M.,Ren, R.,Wang, H.,He, M.,Sun, Y.,Yang, N.,Xu, R.M.
N alpha-acetylated Sir3 stabilizes the conformation of a nucleosome-binding loop in the BAH domain.
Nat.Struct.Mol.Biol., 20:1116-1118, 2013

PubMed Abstract: In Saccharomyces cerevisiae, acetylation of the Sir3 N terminus is important for transcriptional silencing. This covalent modification promotes the binding of the Sir3 BAH domain to the nucleosome, but a mechanistic understanding of this phenomenon is lacking. By X-ray crystallography, we show here that the acetylated N terminus of Sir3 does not interact with the nucleosome directly. Instead, it stabilizes a nucleosome-binding loop in the BAH domain.

PubMed: 23934152
DOI: 10.1038/nsmb.2637

実験手法

X-RAY DIFFRACTION (2.62 Å)