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4KTD

Fab fragment of HIV vaccine-elicited CD4bs-directed antibody, GE136, from non-human primate

Summary for 4KTD
Entry DOI10.2210/pdb4ktd/pdb
Related4KTE
DescriptorGE136 Heavy Chain Fab, GE136 Light Chain Fab, SULFATE ION, ... (5 entities in total)
Functional Keywordsantibody affinity, antibody specificity, vaccine elicited antibodies, fab fragment, aids vaccines, immune system
Biological sourceMacaca mulatta (rhesus macaque)
More
Total number of polymer chains2
Total formula weight48477.89
Authors
Poulsen, C.,Tran, K.,Standfield, R.,Wyatt, R.T. (deposition date: 2013-05-20, release date: 2014-02-05, Last modification date: 2024-10-16)
Primary citationTran, K.,Poulsen, C.,Guenaga, J.,de Val Alda, N.,Wilson, R.,Sundling, C.,Li, Y.,Stanfield, R.L.,Wilson, I.A.,Ward, A.B.,Karlsson Hedestam, G.B.,Wyatt, R.T.
Vaccine-elicited primate antibodies use a distinct approach to the HIV-1 primary receptor binding site informing vaccine redesign.
Proc.Natl.Acad.Sci.USA, 111:E738-E747, 2014
Cited by
PubMed Abstract: HIV-1 neutralization requires Ab accessibility to the functional envelope glycoprotein (Env) spike. We recently reported the isolation of previously unidentified vaccine-elicited, CD4 binding site (CD4bs)-directed mAbs from rhesus macaques immunized with soluble Env trimers, indicating that this region is immunogenic in the context of subunit vaccination. To elucidate the interaction of the trimer-elicited mAbs with gp120 and their insufficient interaction with the HIV-1 primary isolate spike, we crystallized the Fab fragments of two mAbs, GE136 and GE148. Alanine scanning of their complementarity-determining regions, coupled with epitope scanning of their epitopes on gp120, revealed putative contact residues at the Ab/gp120 interface. Docking of the GE136 and GE148 Fabs to gp120, coupled with EM reconstructions of these nonbroadly neutralizing mAbs (non-bNAbs) binding to gp120 monomers and EM modeling to well-ordered trimers, suggested Ab approach to the CD4bs by a vertical angle of access relative to the more lateral mode of interaction used by the CD4bs-directed bNAbs VRC01 and PGV04. Fitting the structures into the available cryo-EM native spike density indicated clashes between these two vaccine-elicited mAbs and the topside variable region spike cap, whereas the bNAbs duck under this quaternary shield to access the CD4bs effectively on primary HIV isolates. These results provide a structural basis for the limited neutralizing breadth observed by current vaccine-induced, CD4bs-directed Abs and highlight the need for better ordered trimer immunogens. The analysis presented here therefore provides valuable information to guide HIV-1 vaccine immunogen redesign.
PubMed: 24550318
DOI: 10.1073/pnas.1319512111
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2 Å)
Structure validation

238582

數據於2025-07-09公開中

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