4KRW

Novel re-arrangement of an RsmA/cSRa family protein to create a structurally distinct new RNA-binding family member

4KRW の概要

• エントリーDOI: 10.2210/pdb4krw/pdb
• 関連するPDBエントリー: 4KJI
• 分子名称: RsmN, a RNA-binding protein of Regulator of Secondary Metabolism, IODIDE ION (3 entities in total)
• 機能のキーワード: beta-barrel, post-transcriptional regulation, rna, rna binding protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9021.92

構造登録者

Li, C. (登録日: 2013-05-17, 公開日: 2013-09-04, 最終更新日: 2024-02-28)

主引用文献

Morris, E.R.,Hall, G.,Li, C.,Heeb, S.,Kulkarni, R.V.,Lovelock, L.,Silistre, H.,Messina, M.,Camara, M.,Emsley, J.,Williams, P.,Searle, M.S.
Structural Rearrangement in an RsmA/CsrA Ortholog of Pseudomonas aeruginosa Creates a Dimeric RNA-Binding Protein, RsmN.
Structure, 21:1659-1671, 2013

PubMed Abstract: In bacteria, the highly conserved RsmA/CsrA family of RNA-binding proteins functions as global posttranscriptional regulators acting on mRNA translation and stability. Through phenotypic complementation of an rsmA mutant in Pseudomonas aeruginosa, we discovered a family member, termed RsmN. Elucidation of the RsmN crystal structure and that of the complex with a hairpin from the sRNA, RsmZ, reveals a uniquely inserted α helix, which redirects the polypeptide chain to form a distinctly different protein fold to the domain-swapped dimeric structure of RsmA homologs. The overall β sheet structure required for RNA recognition is, however, preserved with compensatory sequence and structure differences, allowing the RsmN dimer to target binding motifs in both structured hairpin loops and flexible disordered RNAs. Phylogenetic analysis indicates that, although RsmN appears unique to P. aeruginosa, homologous proteins with the inserted α helix are more widespread and arose as a consequence of a gene duplication event.

PubMed: 23954502
DOI: 10.1016/j.str.2013.07.007

実験手法

X-RAY DIFFRACTION (2.01 Å)