4KPI

Rotational order-disorder structure of reversibly photoswitchable red fluorescent protein rsTagRFP

4KPI の概要

• エントリーDOI: 10.2210/pdb4kpi/pdb
• 関連するPDBエントリー: 3U8A 3U8C
• 分子名称: Reversibly photoswitchable red fluorescent protein rsTagRFP (2 entities in total)
• 機能のキーワード: beta-barrel, order-disorder structure, red fluorescent protein, reversibly photoswitchable fluorescent protein, fluorescent protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27639.46

構造登録者

Pletnev, S. (登録日: 2013-05-13, 公開日: 2014-02-26, 最終更新日: 2023-12-06)

主引用文献

Pletnev, S.,Subach, F.V.,Verkhusha, V.V.,Dauter, Z.
The rotational order-disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP.
Acta Crystallogr.,Sect.D, 70:31-39, 2014

PubMed Abstract: The rotational order-disorder (OD) structure of the reversibly photoswitchable fluorescent protein rsTagRFP is discussed in detail. The structure is composed of tetramers of 222 symmetry incorporated into the lattice in two different orientations rotated 90° with respect to each other around the crystal c axis and with tetramer axes coinciding with the crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates the rotational OD structure with statistically averaged I422 symmetry. Despite order-disorder pathology, the structure of rsTagRFP has electron-density maps of good quality for both non-overlapping and overlapping parts of the model. The crystal contacts, crystal internal architecture and a possible mechanism of rotational OD crystal formation are discussed.

PubMed: 24419376
DOI: 10.1107/S1399004713024644

実験手法

X-RAY DIFFRACTION (1.58 Å)