4KI2

Crystallographic analysis of an RNA-polymerase sigma-subunit fragment complexed with -10 promoter element ssDNA

「4GOR」から置き換えられました

4KI2 の概要

• エントリーDOI: 10.2210/pdb4ki2/pdb
• 関連するPDBエントリー: 3UGO 3UGP
• 分子名称: DNA (5'-D(*TP*GP*TP*AP*CP*AP*AP*TP*GP*GP*G)-3'), RNA polymerase sigma factor, POTASSIUM ION (3 entities in total)
• 機能のキーワード: ssdna, g-quartet, g-quadruplex, promoter recognition, promoter opening, transcription initiation, protein-dna binding, ssdna-binding, transcription-dna complex, transcription/dna
• 由来する生物種: Thermus aquaticus; 詳細
• 細胞内の位置: Cytoplasm : Q9EZJ8
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 69925.27

構造登録者

Feklistov, A.,Darst, S.A. (登録日: 2013-05-01, 公開日: 2013-09-04, 最終更新日: 2024-02-28)

主引用文献

Feklistov, A.,Darst, S.A.
Crystallographic analysis of an RNA polymerase sigma-subunit fragment complexed with -10 promoter element ssDNA: quadruplex formation as a possible tool for engineering crystal contacts in protein-ssDNA complexes.
Acta Crystallogr.,Sect.F, 69:950-955, 2013

PubMed Abstract: Structural studies of -10 promoter element recognition by domain 2 of the RNA polymerase σ subunit [Feklistov & Darst (2011), Cell, 147, 1257-1269] reveal an unusual crystal-packing arrangement dominated by G-quartets. The 3'-terminal GGG motif of the oligonucleotide used in crystallization participates in G-quadruplex formation with GGG motifs from symmetry-related complexes. Stacking between neighboring G-quadruplexes results in the formation of pseudo-continuous four-stranded columns running throughout the length of the crystal (G-columns). Here, a new crystal form is presented with a different arrangement of G-columns and it is proposed that the fortuitous finding of G-quartet packing could be useful in engineering crystal contacts in protein-ssDNA complexes.

PubMed: 23989139
DOI: 10.1107/S1744309113020368

実験手法

X-RAY DIFFRACTION (3.3 Å)