4KGD

High-resolution crystal structure of pyruvate oxidase from L. plantarum in complex with phosphate

4KGD の概要

• エントリーDOI: 10.2210/pdb4kgd/pdb
• 関連するPDBエントリー: 4FEE
• 分子名称: Pyruvate oxidase, FLAVIN-ADENINE DINUCLEOTIDE, MAGNESIUM ION, ... (8 entities in total)
• 機能のキーワード: carbanion, structure activity relationship, oxidation-reduction, umpolung, thiamine diphosphate, reaction intermediate, oxidoreductase
• 由来する生物種: Lactobacillus plantarum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 135941.82

構造登録者

Neumann, P.,Tittmann, K. (登録日: 2013-04-29, 公開日: 2013-06-12, 最終更新日: 2023-09-20)

主引用文献

Meyer, D.,Neumann, P.,Ficner, R.,Tittmann, K.
Observation of a stable carbene at the active site of a thiamin enzyme.
Nat.Chem.Biol., 9:488-490, 2013

PubMed Abstract: Carbenes are highly reactive chemical compounds that are exploited as ligands in organometallic chemistry and are powerful organic catalysts. They were postulated to occur as transient intermediates in enzymes, yet their existence in a biological system could never be demonstrated directly. We present spectroscopic and structural data of a thiamin enzyme in a noncovalent complex with substrate, which implicate accumulation of a stable carbene as a major resonance contributor to deprotonated thiamin.

PubMed: 23748673
DOI: 10.1038/nchembio.1275

実験手法

X-RAY DIFFRACTION (1.06 Å)