4KF4

Crystal Structure of sfCherry

4KF4 の概要

• エントリーDOI: 10.2210/pdb4kf4/pdb
• 関連するPDBエントリー: 4KF5
• 分子名称: fluorescent protein sfCherry (2 entities in total)
• 機能のキーワード: fluorescent protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 202641.75

構造登録者

Nguyen, H.B.,Hung, L.-W.,Yeates, T.O.,Waldo, G.S.,Terwilliger, T.C. (登録日: 2013-04-26, 公開日: 2013-12-18, 最終更新日: 2024-11-27)

主引用文献

Nguyen, H.B.,Hung, L.W.,Yeates, T.O.,Terwilliger, T.C.,Waldo, G.S.
Split green fluorescent protein as a modular binding partner for protein crystallization.
Acta Crystallogr.,Sect.D, 69:2513-2523, 2013

PubMed Abstract: A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP β-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was tested by inserting this hairpin into a loop of another fluorescent protein, sfCherry. The crystal structure of the sfCherry-GFP(10-11) hairpin in complex with GFP(1-9) was determined at a resolution of 2.6 Å. Analysis of the complex shows that the reconstituted GFP is attached to the target protein (sfCherry) in a structurally ordered way. This work opens the way to rapidly creating crystallization variants by reconstituting a target protein bearing the GFP(10-11) hairpin with a variety of GFP(1-9) mutants engineered for favorable crystallization.

PubMed: 24311592
DOI: 10.1107/S0907444913024608

実験手法

X-RAY DIFFRACTION (1.994 Å)