4KDT

Structure of an early native-like intermediate of beta2-microglobulin amyloidosis

4KDT の概要

• エントリーDOI: 10.2210/pdb4kdt/pdb
• 分子名称: Nanobody24, Beta-2-microglobulin, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: immunoglobulin fold, immune system
• 由来する生物種: Camelidae; 詳細
• 細胞内の位置: Secreted . Note=(Microbial infection) In the presence of M: P61769
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 54422.11

構造登録者

Vanderhaegen, S.,Fislage, M.,Pardon, E.,Versees, W.,Steyaert, J. (登録日: 2013-04-25, 公開日: 2013-08-28, 最終更新日: 2024-11-20)

主引用文献

Vanderhaegen, S.,Fislage, M.,Domanska, K.,Versees, W.,Pardon, E.,Bellotti, V.,Steyaert, J.
Structure of an early native-like intermediate of beta 2-microglobulin amyloidogenesis.
Protein Sci., 22:1349-1357, 2013

PubMed Abstract: To investigate early intermediates of β2-microglobulin (β2m) amyloidogenesis, we solved the structure of β2m containing the amyloidogenic Pro32Gly mutation by X-ray crystallography. One nanobody (Nb24) that efficiently blocks fibril elongation was used as a chaperone to co-crystallize the Pro32Gly β2m monomer under physiological conditions. The complex of P32G β2m with Nb24 reveals a trans peptide bond at position 32 of this amyloidogenic variant, whereas Pro32 adopts the cis conformation in the wild-type monomer, indicating that the cis to trans isomerization at Pro32 plays a critical role in the early onset of β2m amyloid formation.

PubMed: 23904325
DOI: 10.1002/pro.2321

実験手法

X-RAY DIFFRACTION (2.6 Å)