4K59

Crystal Structure of Pseudomonas Aeruginosa RsmF

4K59 の概要

• エントリーDOI: 10.2210/pdb4k59/pdb
• 分子名称: RNA BINDING PROTEIN RsmF (2 entities in total)
• 機能のキーワード: beta sandwich, rna binding protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8067.35

構造登録者

Betts, L.,Walton, W.G.,Redinbo, M.R. (登録日: 2013-04-14, 公開日: 2013-08-28, 最終更新日: 2024-02-28)

主引用文献

Marden, J.N.,Diaz, M.R.,Walton, W.G.,Gode, C.J.,Betts, L.,Urbanowski, M.L.,Redinbo, M.R.,Yahr, T.L.,Wolfgang, M.C.
An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses in Pseudomonas aeruginosa.
Proc.Natl.Acad.Sci.USA, 110:15055-15060, 2013

PubMed Abstract: Members of the CsrA family of prokaryotic mRNA-binding proteins alter the translation and/or stability of transcripts needed for numerous global physiological processes. The previously described CsrA family member in Pseudomonas aeruginosa (RsmA) plays a central role in determining infection modality by reciprocally regulating processes associated with acute (type III secretion and motility) and chronic (type VI secretion and biofilm formation) infection. Here we describe a second, structurally distinct RsmA homolog in P. aeruginosa (RsmF) that has an overlapping yet unique regulatory role. RsmF deviates from the canonical 5 β-strand and carboxyl-terminal α-helix topology of all other CsrA proteins by having the α-helix internally positioned. Despite striking changes in topology, RsmF adopts a tertiary structure similar to other CsrA family members and binds a subset of RsmA mRNA targets, suggesting that RsmF activity is mediated through a conserved mechanism of RNA recognition. Whereas deletion of rsmF alone had little effect on RsmA-regulated processes, strains lacking both rsmA and rsmF exhibited enhanced RsmA phenotypes for markers of both type III and type VI secretion systems. In addition, simultaneous deletion of rsmA and rsmF resulted in superior biofilm formation relative to the wild-type or rsmA strains. We show that RsmF translation is derepressed in an rsmA mutant and demonstrate that RsmA specifically binds to rsmF mRNA in vitro, creating a global hierarchical regulatory cascade that operates at the posttranscriptional level.

PubMed: 23980177
DOI: 10.1073/pnas.1307217110

実験手法

X-RAY DIFFRACTION (2.22 Å)