4K2E

HlyU from Vibrio cholerae N16961

4K2E の概要

• エントリーDOI: 10.2210/pdb4k2e/pdb
• 分子名称: Transcriptional activator HlyU (2 entities in total)
• 機能のキーワード: winged helix, dna-binding domain, hemolysin gene transcription regulator, dna, s-hydroxycysteine, transcription activator
• 由来する生物種: Vibrio cholerae
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 50951.32

構造登録者

Mukherjee, D.,Datta, A.B.,Chakrabarti, P. (登録日: 2013-04-09, 公開日: 2014-04-30, 最終更新日: 2024-11-13)

主引用文献

Mukherjee, D.,Datta, A.B.,Chakrabarti, P.
Crystal structure of HlyU, the hemolysin gene transcription activator, from Vibrio cholerae N16961 and functional implications.
Biochim.Biophys.Acta, 1844:2346-2354, 2014

PubMed Abstract: HlyU in Vibrio cholerae is known to be the transcriptional activator of the hemolysin gene, HlyA and possibly a regulator of other virulence factors influencing growth, colonization and pathogenicity of this infective agent. Here we report the crystal structure of HlyU from V. cholerae N16961 (HlyU_Vc) at 1.8Å. The protein, with five α-helices and three β-strands in the topology of α1-α2-β1-α3-α4-β2-β3-α5, forms a homodimer. Helices α3-α4 and a β sheet form the winged helix-turn-helix (wHTH) DNA-binding motif common to the transcription regulators of the SmtB/ArsR family. In spite of an overall fold similar to SmtB/ArsR family, it lacks any metal binding site seen in SmtB. A comparison of the dimeric interfaces showed that the one in SmtB is much larger and have salt bridges that can be disrupted to accommodate metal ions. A model of HlyU-DNA complex suggests bending of the DNA. Cys38 in the structure was found to be modified as sulfenic acid; the oxidized form was not seen in another structure solved under reducing condition. Although devoid of any metal binding site, the presence of a Cys residue exhibiting oxidation-reduction suggests the possibility of the existence of a redox switch in transcription regulation. A structure-based phylogenetic analysis of wHTH proteins revealed the segregation of metal and non-metal binding proteins as well as those in the latter group that are under redox control.

PubMed: 25450504
DOI: 10.1016/j.bbapap.2014.09.020

実験手法

X-RAY DIFFRACTION (1.8 Å)