4K1C

VCX1 Calcium/Proton Exchanger

4K1C の概要

• エントリーDOI: 10.2210/pdb4k1c/pdb
• 分子名称: Vacuolar calcium ion transporter, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: structural genomics, psi-biology, protein structure initiative, center for structures of membrane proteins, csmp, membrane protein cax ncx nckx, calcium transport, vacuole, transport protein, membrane protein-metal transport complex, membrane protein/metal transport
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• 細胞内の位置: Vacuole membrane; Multi-pass membrane protein: Q99385
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 95310.94

構造登録者

Waight, A.B.,Pedersen, B.P.,Stroud, R.M.,Center for Structures of Membrane Proteins (CSMP) (登録日: 2013-04-04, 公開日: 2013-05-08, 最終更新日: 2024-02-28)

主引用文献

Waight, A.B.,Pedersen, B.P.,Schlessinger, A.,Bonomi, M.,Chau, B.H.,Roe-Zurz, Z.,Risenmay, A.J.,Sali, A.,Stroud, R.M.
Structural basis for alternating access of a eukaryotic calcium/proton exchanger.
Nature, 499:107-110, 2013

PubMed Abstract: Eukaryotic Ca(2+) regulation involves sequestration into intracellular organelles, and expeditious Ca(2+) release into the cytosol is a hallmark of key signalling transduction pathways. Bulk removal of Ca(2+) after such signalling events is accomplished by members of the Ca(2+):cation (CaCA) superfamily. The CaCA superfamily includes the Na(+)/Ca(2+) (NCX) and Ca(2+)/H(+) (CAX) antiporters, and in mammals the NCX and related proteins constitute families SLC8 and SLC24, and are responsible for the re-establishment of Ca(2+) resting potential in muscle cells, neuronal signalling and Ca(2+) reabsorption in the kidney. The CAX family members maintain cytosolic Ca(2+) homeostasis in plants and fungi during steep rises in intracellular Ca(2+) due to environmental changes, or following signal transduction caused by events such as hyperosmotic shock, hormone response and response to mating pheromones. The cytosol-facing conformations within the CaCA superfamily are unknown, and the transport mechanism remains speculative. Here we determine a crystal structure of the Saccharomyces cerevisiae vacuolar Ca(2+)/H(+) exchanger (Vcx1) at 2.3 Å resolution in a cytosol-facing, substrate-bound conformation. Vcx1 is the first structure, to our knowledge, within the CAX family, and it describes the key cytosol-facing conformation of the CaCA superfamily, providing the structural basis for a novel alternating access mechanism by which the CaCA superfamily performs high-throughput Ca(2+) transport across membranes.

PubMed: 23685453
DOI: 10.1038/nature12233

実験手法

X-RAY DIFFRACTION (2.3 Å)