4K08

Periplasmic sensor domain of chemotaxis protein, Adeh_3718

4K08 の概要

• エントリーDOI: 10.2210/pdb4k08/pdb
• 分子名称: Methyl-accepting chemotaxis sensory transducer, ZINC ION, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: methyl accepting chemotaxis, anaeromyxobacter dehalogenans, pas-like sensor domain, structural genomics, psi-biology, midwest center for structural genomics, mcsg, periplasmic sensor domain, signaling protein
• 由来する生物種: Anaeromyxobacter dehalogenans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17441.48

構造登録者

Pokkuluri, P.R.,Mack, J.C.,Bearden, J.,Rakowski, E.,Schiffer, M.,Joachimiak, A.,Midwest Center for Structural Genomics (MCSG) (登録日: 2013-04-03, 公開日: 2013-07-17, 最終更新日: 2015-04-15)

主引用文献

Pokkuluri, P.R.,Dwulit-Smith, J.,Duke, N.E.,Wilton, R.,Mack, J.C.,Bearden, J.,Rakowski, E.,Babnigg, G.,Szurmant, H.,Joachimiak, A.,Schiffer, M.
Analysis of periplasmic sensor domains from Anaeromyxobacter dehalogenans 2CP-C: structure of one sensor domain from a histidine kinase and another from a chemotaxis protein.
Microbiologyopen, 2:766-777, 2013

PubMed Abstract: Anaeromyxobacter dehalogenans is a δ-proteobacterium found in diverse soils and sediments. It is of interest in bioremediation efforts due to its dechlorination and metal-reducing capabilities. To gain an understanding on A. dehalogenans' abilities to adapt to diverse environments we analyzed its signal transduction proteins. The A. dehalogenans genome codes for a large number of sensor histidine kinases (HK) and methyl-accepting chemotaxis proteins (MCP); among these 23 HK and 11 MCP proteins have a sensor domain in the periplasm. These proteins most likely contribute to adaptation to the organism's surroundings. We predicted their three-dimensional folds and determined the structures of two of the periplasmic sensor domains by X-ray diffraction. Most of the domains are predicted to have either PAS-like or helical bundle structures, with two predicted to have solute-binding protein fold, and another predicted to have a 6-phosphogluconolactonase like fold. Atomic structures of two sensor domains confirmed the respective fold predictions. The Adeh_2942 sensor (HK) was found to have a helical bundle structure, and the Adeh_3718 sensor (MCP) has a PAS-like structure. Interestingly, the Adeh_3718 sensor has an acetate moiety bound in a binding site typical for PAS-like domains. Future work is needed to determine whether Adeh_3718 is involved in acetate sensing by A. dehalogenans.

PubMed: 23897711
DOI: 10.1002/mbo3.112

実験手法

X-RAY DIFFRACTION (2 Å)