4JYA
Crystal structures of pseudouridinilated stop codons with ASLs
Summary for 4JYA
| Entry DOI | 10.2210/pdb4jya/pdb |
| Related | 4JV5 |
| Descriptor | 16S ribosomal RNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (25 entities in total) |
| Functional Keywords | protein synthesis, ribosome |
| Biological source | Thermus thermophilus More |
| Total number of polymer chains | 23 |
| Total formula weight | 770137.69 |
| Authors | Fernandez, I.S.,Ng, C.L.,Kelley, A.C.,Guowei, W.,Yu, Y.T.,Ramakrishnan, V. (deposition date: 2013-03-29, release date: 2013-06-26, Last modification date: 2024-11-27) |
| Primary citation | Fernandez, I.S.,Ng, C.L.,Kelley, A.C.,Wu, G.,Yu, Y.T.,Ramakrishnan, V. Unusual base pairing during the decoding of a stop codon by the ribosome. Nature, 500:107-110, 2013 Cited by PubMed Abstract: During normal translation, the binding of a release factor to one of the three stop codons (UGA, UAA or UAG) results in the termination of protein synthesis. However, modification of the initial uridine to a pseudouridine (Ψ) allows efficient recognition and read-through of these stop codons by a transfer RNA (tRNA), although it requires the formation of two normally forbidden purine-purine base pairs. Here we determined the crystal structure at 3.1 Å resolution of the 30S ribosomal subunit in complex with the anticodon stem loop of tRNA(Ser) bound to the ΨAG stop codon in the A site. The ΨA base pair at the first position is accompanied by the formation of purine-purine base pairs at the second and third positions of the codon, which show an unusual Watson-Crick/Hoogsteen geometry. The structure shows a previously unsuspected ability of the ribosomal decoding centre to accommodate non-canonical base pairs. PubMed: 23812587DOI: 10.1038/nature12302 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.098 Å) |
Structure validation
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