4JYA

Crystal structures of pseudouridinilated stop codons with ASLs

4JYA の概要

• エントリーDOI: 10.2210/pdb4jya/pdb
• 関連するPDBエントリー: 4JV5
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (25 entities in total)
• 機能のキーワード: protein synthesis, ribosome
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 770137.69

構造登録者

Fernandez, I.S.,Ng, C.L.,Kelley, A.C.,Guowei, W.,Yu, Y.T.,Ramakrishnan, V. (登録日: 2013-03-29, 公開日: 2013-06-26, 最終更新日: 2024-11-27)

主引用文献

Fernandez, I.S.,Ng, C.L.,Kelley, A.C.,Wu, G.,Yu, Y.T.,Ramakrishnan, V.
Unusual base pairing during the decoding of a stop codon by the ribosome.
Nature, 500:107-110, 2013

PubMed Abstract: During normal translation, the binding of a release factor to one of the three stop codons (UGA, UAA or UAG) results in the termination of protein synthesis. However, modification of the initial uridine to a pseudouridine (Ψ) allows efficient recognition and read-through of these stop codons by a transfer RNA (tRNA), although it requires the formation of two normally forbidden purine-purine base pairs. Here we determined the crystal structure at 3.1 Å resolution of the 30S ribosomal subunit in complex with the anticodon stem loop of tRNA(Ser) bound to the ΨAG stop codon in the A site. The ΨA base pair at the first position is accompanied by the formation of purine-purine base pairs at the second and third positions of the codon, which show an unusual Watson-Crick/Hoogsteen geometry. The structure shows a previously unsuspected ability of the ribosomal decoding centre to accommodate non-canonical base pairs.

PubMed: 23812587
DOI: 10.1038/nature12302

実験手法

X-RAY DIFFRACTION (3.098 Å)