4JWS

Crystal structure of Cytochrome P450cam-putidaredoxin complex

4JWS の概要

• エントリーDOI: 10.2210/pdb4jws/pdb
• 関連するPDBエントリー: 4JWU 4JX1
• 分子名称: Camphor 5-monooxygenase, Putidaredoxin, PROTOPORPHYRIN IX CONTAINING FE, ... (7 entities in total)
• 機能のキーワード: p450cam-pdx complex, redox partners, oxidoreductase-electron transport complex, oxidoreductase/electron transport
• 由来する生物種: Pseudomonas putida; 詳細
• 細胞内の位置: Cytoplasm (By similarity): P00183
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 119936.42

構造登録者

Tripathi, S.M.,Li, H.,Poulos, T.L. (登録日: 2013-03-27, 公開日: 2013-06-19, 最終更新日: 2024-10-16)

主引用文献

Tripathi, S.,Li, H.,Poulos, T.L.
Structural basis for effector control and redox partner recognition in cytochrome P450.
Science, 340:1227-1230, 2013

PubMed Abstract: Cytochromes P450 catalyze a variety of monooxygenase reactions that require electron transfer from redox partners. Although the structure of many P450s and a small handful of redox partners are known, there is very little structural information available on redox complexes, thus leaving a gap in our understanding on the control of P450-redox partner interactions. We have solved the crystal structure of oxidized and reduced P450cam complexed with its redox partner, putidaredoxin (Pdx), to 2.2 and 2.09 angstroms, respectively. It was anticipated that Pdx would favor closed substrate-bound P450cam, which differs substantially from the open conformer, but instead we found that Pdx favors the open state. These new structures indicate that the effector role of Pdx is to shift P450cam toward the open conformation, which enables the establishment of a water-mediated H-bonded network, which is required for proton-coupled electron transfer.

PubMed: 23744947
DOI: 10.1126/science.1235797

実験手法

X-RAY DIFFRACTION (2.15 Å)