4JRT

Crystal structure of an A-form RNA duplex containing three GU base pairs

4JRT の概要

• エントリーDOI: 10.2210/pdb4jrt/pdb
• 分子名称: RNA (5'-R(P*GP*GP*GP*UP*GP*GP*UP*GP*CP*GP*GP*G)-3'), RNA (5'-R(P*CP*CP*UP*GP*CP*AP*CP*UP*GP*CP*CP*C)-3') (3 entities in total)
• 機能のキーワード: a-form rna, ribose-zipper motif, gu base pair, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7702.67

構造登録者

Kondo, J.,Dock-Bregeon, A.C.,Willkomm, D.K.,Hartmann, R.K.,Westhof, E. (登録日: 2013-03-21, 公開日: 2013-06-05, 最終更新日: 2023-11-08)

主引用文献

Kondo, J.,Dock-Bregeon, A.C.,Willkomm, D.K.,Hartmann, R.K.,Westhof, E.
Structure of an A-form RNA duplex obtained by degradation of 6S RNA in a crystallization droplet
Acta Crystallogr.,Sect.F, 69:634-639, 2013

PubMed Abstract: In the course of a crystallographic study of a 132 nt variant of Aquifex aeolicus 6S RNA, a crystal structure of an A-form RNA duplex containing 12 base pairs was solved at a resolution of 2.6 Å. In fact, the RNA duplex is part of the 6S RNA and was obtained by accidental but precise degradation of the 6S RNA in a crystallization droplet. 6S RNA degradation was confirmed by microscopic observation of crystals and gel electrophoresis of crystallization droplets. The RNA oligomers obtained form regular A-form duplexes containing three GoU wobble-type base pairs, one of which engages in intermolecular contacts through a ribose-zipper motif at the crystal-packing interface.

PubMed: 23722840
DOI: 10.1107/S1744309113013018

実験手法

X-RAY DIFFRACTION (2.6 Å)