4JRN

ROP18 kinase domain in complex with AMP-PNP and sucrose

4JRN の概要

• エントリーDOI: 10.2210/pdb4jrn/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900003
• 分子名称: Rhoptry kinase family protein, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (5 entities in total)
• 機能のキーワード: protein kinase, kinase, membrane, transferase
• 由来する生物種: Toxoplasma gondii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42403.27

構造登録者

Lim, D.,Gold, D.A.,Lindsay, J.,Rosowski, E.E.,Niedelman, W.,Yaffe, M.B.,Saeij, J.P.J. (登録日: 2013-03-21, 公開日: 2013-10-23, 最終更新日: 2024-11-27)

主引用文献

Lim, D.,Gold, D.A.,Julien, L.,Rosowski, E.E.,Niedelman, W.,Yaffe, M.B.,Saeij, J.P.
Structure of the Toxoplasma gondii ROP18 kinase domain reveals a second ligand binding pocket required for acute virulence.
J.Biol.Chem., 288:34968-34980, 2013

PubMed Abstract: At least a third of the human population is infected with the intracellular parasite Toxoplasma gondii, which contributes significantly to the disease burden in immunocompromised and neutropenic hosts and causes serious congenital complications when vertically transmitted to the fetus. Genetic analyses have identified the Toxoplasma ROP18 Ser/Thr protein kinase as a major factor mediating acute virulence in mice. ROP18 is secreted into the host cell during the invasion process, and its catalytic activity is required for the acute virulence phenotype. However, its precise molecular function and regulation are not fully understood. We have determined the crystal structure of the ROP18 kinase domain, which is inconsistent with a previously proposed autoinhibitory mechanism of regulation. Furthermore, a sucrose molecule bound to our structure identifies an additional ligand-binding pocket outside of the active site cleft. Mutational analysis confirms an important role for this pocket in virulence.

PubMed: 24129568
DOI: 10.1074/jbc.M113.523266

実験手法

X-RAY DIFFRACTION (2.71 Å)