4JRC

Distal Stem I region from G. kaustophilus glyQS T box RNA

4JRC の概要

• エントリーDOI: 10.2210/pdb4jrc/pdb
• 分子名称: Distal Stem I region of the glyQS T box leader RNA, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: t box rna, regulatory rna leader sequence, trna binding, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37358.61

構造登録者

Grigg, J.C.,Ke, A. (登録日: 2013-03-21, 公開日: 2013-04-24, 最終更新日: 2024-02-28)

主引用文献

Grigg, J.C.,Chen, Y.,Grundy, F.J.,Henkin, T.M.,Pollack, L.,Ke, A.
T box RNA decodes both the information content and geometry of tRNA to affect gene expression.
Proc.Natl.Acad.Sci.USA, 110:7240-7245, 2013

PubMed Abstract: The T box leader sequence is an RNA element that controls gene expression by binding directly to a specific tRNA and sensing its aminoacylation state. This interaction controls expression of amino acid-related genes in a negative feedback loop. The T box RNA structure is highly conserved, but its tRNA binding mechanism is only partially understood. Known sequence elements are the specifier sequence, which recognizes the tRNA anticodon, and the antiterminator bulge, which base pairs with the tRNA acceptor end. Here, we reveal the crucial function of the highly conserved stem I distal region in tRNA recognition and report its 2.65-Å crystal structure. The apex of this region contains an intricately woven loop-loop interaction between two conserved motifs, the Adenine-guanine (AG) bulge and the distal loop. This loop-loop structure presents a base triple on its surface that is optimally positioned for base-stacking interactions. Mutagenesis, cross-linking, and small-angle X-ray scattering data demonstrate that the apical base triple serves as a binding platform to dock the tRNA D- and T-loops. Strikingly, the binding platform strongly resembles the D- and T-loop binding elements from RNase P and the ribosome exit site, suggesting that this loop-loop structure may represent a widespread tRNA recognition platform. We propose a two-checkpoint molecular ruler model for tRNA decoding in which the information content of tRNA is first examined through specifier sequence-anticodon interaction, and the length of the tRNA anticodon arm is then measured by the distal loop-loop platform. When both conditions are met, tRNA is secured, and its aminoacylation state is sensed.

PubMed: 23589841
DOI: 10.1073/pnas.1222214110

実験手法

X-RAY DIFFRACTION (2.673 Å)