4JMU

Crystal structure of HIV matrix residues 1-111 in complex with inhibitor

4JMU の概要

• エントリーDOI: 10.2210/pdb4jmu/pdb
• 分子名称: Gag-Pol polyprotein, SULFATE ION, 5-{4-[(4-methoxybenzoyl)amino]phenoxy}-2-{[(trans-4-methylcyclohexyl)carbonyl](propan-2-yl)amino}benzoic acid, ... (4 entities in total)
• 機能のキーワード: structural protein matrix, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Human immunodeficiency virus type 1 (NEW YORK-5 ISOLATE) (HIV-1)
• 細胞内の位置: Matrix protein p17: Virion (Potential). Capsid protein p24: Virion (Potential). Nucleocapsid protein p7: Virion (Potential). Reverse transcriptase/ribonuclease H: Virion (Potential). Integrase: Virion (Potential): P12497
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13408.28

構造登録者

Lemke, C.T. (登録日: 2013-03-14, 公開日: 2013-10-30, 最終更新日: 2024-02-28)

主引用文献

Laplante, S.R.,Forgione, P.,Boucher, C.,Coulombe, R.,Gillard, J.,Hucke, O.,Jakalian, A.,Joly, M.A.,Kukolj, G.,Lemke, C.,McCollum, R.,Titolo, S.,Beaulieu, P.L.,Stammers, T.
Enantiomeric Atropisomers Inhibit HCV Polymerase and/or HIV Matrix: Characterizing Hindered Bond Rotations and Target Selectivity.
J.Med.Chem., 57:1944-1951, 2014

PubMed Abstract: An anthranilic acid series of allosteric thumb pocket 2 HCV NS5B polymerase inhibitors exhibited hindered rotation along a covalent bond axis, and the existence of atropisomer chirality was confirmed by NMR, HPLC analysis on chiral supports, and computational studies. A thorough understanding of the concerted rotational properties and the influence exerted by substituents involved in this steric phenomenon was attained through biophysical studies on a series of truncated analogues. The racemization half-life of a compound within this series was determined to be 69 min, which was consistent with a class 2 atropisomer (intermediate conformational exchange). It was further found by X-ray crystallography that one enantiomer of a compound bound to the intended HCV NS5B polymerase target whereas the mirror image atropisomer was able to bind to an unrelated HIV matrix target. Analogues were then identified that selectively inhibited the former. These studies highlight that atropisomer chirality can lead to distinct entities with specific properties, and the phenomenon of atropisomerism in drug discovery should be evaluated and appropriately managed.

PubMed: 24024973
DOI: 10.1021/jm401202a

実験手法

X-RAY DIFFRACTION (2 Å)