4JLQ

Crystal structure of human Karyopherin-beta2 bound to the PY-NLS of Saccharomyces cerevisiae NAB2

「4H1K」から置き換えられました

4JLQ の概要

• エントリーDOI: 10.2210/pdb4jlq/pdb
• 分子名称: Transportin-1, Nuclear polyadenylated RNA-binding protein NAB2 (2 entities in total)
• 機能のキーワード: heat repeats, karyopherin, nuclear import, proteintransport, importin, transportin, transport protein, nls, nab2, structural genomics, nucleocytoplasmic transport: a target for cellular control (npcxstals), new york structural genomics research consortium (nysgrc), psi-biology, npcxstals
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: Q92973; Nucleus: P32505
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 100790.23

構造登録者

Sampathkumar, P.,Gizzi, A.,Rout, M.P.,Chook, Y.M.,Almo, S.C.,New York Structural Genomics Research Consortium (NYSGRC),Nucleocytoplasmic Transport: a Target for Cellular Control (NPCXstals) (登録日: 2013-03-12, 公開日: 2013-04-03, 最終更新日: 2023-09-20)

主引用文献

Soniat, M.,Sampathkumar, P.,Collett, G.,Gizzi, A.S.,Banu, R.N.,Bhosle, R.C.,Chamala, S.,Chowdhury, S.,Fiser, A.,Glenn, A.S.,Hammonds, J.,Hillerich, B.,Khafizov, K.,Love, J.D.,Matikainen, B.,Seidel, R.D.,Toro, R.,Rajesh Kumar, P.,Bonanno, J.B.,Chook, Y.M.,Almo, S.C.
Crystal structure of human Karyopherin beta 2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2.
J.Struct.Funct.Genom., 14:31-35, 2013

PubMed Abstract: Import-Karyopherin or Importin proteins bind nuclear localization signals (NLSs) to mediate the import of proteins into the cell nucleus. Karyopherin β2 or Kapβ2, also known as Transportin, is a member of this transporter family responsible for the import of numerous RNA binding proteins. Kapβ2 recognizes a targeting signal termed the PY-NLS that lies within its cargos to target them through the nuclear pore complex. The recognition of PY-NLS by Kapβ2 is conserved throughout eukaryotes. Kap104, the Kapβ2 homolog in Saccharomyces cerevisiae, recognizes PY-NLSs in cargos Nab2, Hrp1, and Tfg2. We have determined the crystal structure of Kapβ2 bound to the PY-NLS of the mRNA processing protein Nab2 at 3.05-Å resolution. A seven-residue segment of the PY-NLS of Nab2 is observed to bind Kapβ2 in an extended conformation and occupies the same PY-NLS binding site observed in other Kapβ2·PY-NLS structures.

PubMed: 23535894
DOI: 10.1007/s10969-013-9150-1

実験手法

X-RAY DIFFRACTION (3.05 Å)