4JI5

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

4JI5 の概要

• エントリーDOI: 10.2210/pdb4ji5/pdb
• 関連するPDBエントリー: 4JI0 4JI1 4JI2 4JI3 4JI4 4JI6 4JI7 4JI8
• 分子名称: 16S rRNA, RIBOSOMAL PROTEIN S10, RIBOSOMAL PROTEIN S11, ... (24 entities in total)
• 機能のキーワード: 30s ribosomal subunit, ribosome, streptomycin, rna structure, thermus thermophilus, antibiotic resistance, decoding, ribosome-antibiotic complex, ribosome/antibiotic
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 21
• 化学式量合計: 788247.86

構造登録者

Demirci, H.,Wang, L.,Murphy IV, F.,Murphy, E.,Carr, J.,Blanchard, S.,Jogl, G.,Dahlberg, A.E.,Gregory, S.T. (登録日: 2013-03-05, 公開日: 2013-11-06, 最終更新日: 2025-02-12)

主引用文献

Demirci, H.,Wang, L.,Murphy, F.V.,Murphy, E.L.,Carr, J.F.,Blanchard, S.C.,Jogl, G.,Dahlberg, A.E.,Gregory, S.T.
The central role of protein S12 in organizing the structure of the decoding site of the ribosome.
Rna, 19:1791-1801, 2013

PubMed Abstract: The ribosome decodes mRNA by monitoring the geometry of codon-anticodon base-pairing using a set of universally conserved 16S rRNA nucleotides within the conformationally dynamic decoding site. By applying single-molecule FRET and X-ray crystallography, we have determined that conditional-lethal, streptomycin-dependence mutations in ribosomal protein S12 interfere with tRNA selection by allowing conformational distortions of the decoding site that impair GTPase activation of EF-Tu during the tRNA selection process. Distortions in the decoding site are reversed by streptomycin or by a second-site suppressor mutation in 16S rRNA. These observations encourage a refinement of the current model for decoding, wherein ribosomal protein S12 and the decoding site collaborate to optimize codon recognition and substrate discrimination during the early stages of the tRNA selection process.

PubMed: 24152548
DOI: 10.1261/rna.040030.113

実験手法

X-RAY DIFFRACTION (3.85 Å)