4JHU
T2-depleted laccase from Coriolopsis caperata soaked with CuCl
Summary for 4JHU
| Entry DOI | 10.2210/pdb4jhu/pdb |
| Related | 4JHV |
| Descriptor | LACCASE, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, COPPER (II) ION, ... (5 entities in total) |
| Functional Keywords | beta sheet, 4-copper protein, metal-binding, oxidoreductase, laccase |
| Biological source | Coriolopsis Caperata |
| Total number of polymer chains | 1 |
| Total formula weight | 55303.70 |
| Authors | Polyakov, K.M.,Fedorova, T.V.,Glazunova, O.A.,Kurzeev, S.A.,Maloshenok, L.G.,Koroleva, O.A. (deposition date: 2013-03-05, release date: 2014-04-23, Last modification date: 2020-07-29) |
| Primary citation | Glazunova, O.A.,Polyakov, K.M.,Fedorova, T.V.,Dorovatovskii, P.V.,Koroleva, O.V. Elucidation of the crystal structure of Coriolopsis caperata laccase: restoration of the structure and activity of the native enzyme from the T2-depleted form by copper ions. Acta Crystallogr.,Sect.D, 71:854-861, 2015 Cited by PubMed Abstract: Laccases are members of a large family of multicopper oxidases that catalyze the oxidation of a wide range of organic and inorganic substrates accompanied by the reduction of dioxygen to water. A new laccase was isolated from the basidiomycete Coriolopsis caperata strain 0677 and its amino-acid sequence was determined. According to its physicochemical properties and spectroscopic features, the laccase from C. caperata is a high redox-potential blue laccase. Attempts to crystallize the native enzyme were unsuccessful. The copper type 2-depleted (T2D) laccase was prepared and crystallized. The structure of T2D laccase from C. caperata was solved at 1.6 Å resolution, and attempts to reconstruct the T2 copper centre were performed using Cu(+) and Cu(2+) ions. The structure of T2D+Cu(+) laccase was solved at 1.89 Å resolution. It was shown that the T2D+Cu(+) laccase structure contained four copper ions in the active site. Reconstruction could not be achieved when the T2D laccase crystals were treated with CuSO4. PubMed: 25849396DOI: 10.1107/S1399004715001595 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.89 Å) |
Structure validation
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