4JGZ

Crystal structure of human coxsackievirus A16 uncoating intermediate (space group I222)

4JGZ の概要

• エントリーDOI: 10.2210/pdb4jgz/pdb
• 分子名称: Polyprotein, capsid protein VP1, Polyprotein, capsid protein VP2, Polyprotein, capsid protein VP3 (3 entities in total)
• 機能のキーワード: virus, hand-foot-and-mouth disease, picornavirus uncoating, pocket factor, icosahedral virus
• 由来する生物種: Human coxsackievirus A16; 詳細
• 細胞内の位置: Picornain 3C: Host cytoplasm (By similarity). Protein 3B: Virion (By similarity): I3W9E1 I3W9E1 I3W9E1
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 87331.78

構造登録者

Ren, J.,Wang, X.,Hu, Z.,Gao, Q.,Sun, Y.,Li, X.,Porta, C.,Walter, T.S.,Gilbert, R.J.,Zhao, Y.,Axford, D.,Williams, M.,McAuley, K.,Rowlands, D.J.,Yin, W.,Wang, J.,Stuart, D.I.,Rao, Z.,Fry, E.E. (登録日: 2013-03-04, 公開日: 2013-06-05, 最終更新日: 2024-11-20)

主引用文献

Ren, J.,Wang, X.,Hu, Z.,Gao, Q.,Sun, Y.,Li, X.,Porta, C.,Walter, T.S.,Gilbert, R.J.,Zhao, Y.,Axford, D.,Williams, M.,McAuley, K.,Rowlands, D.J.,Yin, W.,Wang, J.,Stuart, D.I.,Rao, Z.,Fry, E.E.
Picornavirus uncoating intermediate captured in atomic detail.
Nat Commun, 4:1929-1929, 2013

PubMed Abstract: It remains largely mysterious how the genomes of non-enveloped eukaryotic viruses are transferred across a membrane into the host cell. Picornaviruses are simple models for such viruses, and initiate this uncoating process through particle expansion, which reveals channels through which internal capsid proteins and the viral genome presumably exit the particle, although this has not been clearly seen until now. Here we present the atomic structure of an uncoating intermediate for the major human picornavirus pathogen CAV16, which reveals VP1 partly extruded from the capsid, poised to embed in the host membrane. Together with previous low-resolution results, we are able to propose a detailed hypothesis for the ordered egress of the internal proteins, using two distinct sets of channels through the capsid, and suggest a structural link to the condensed RNA within the particle, which may be involved in triggering RNA release.

PubMed: 23728514
DOI: 10.1038/ncomms2889

実験手法

X-RAY DIFFRACTION (3 Å)