4JGF

Crystal Structure of the Cataract-Causing P23T gamma D-Crystallin Mutant

4JGF の概要

• エントリーDOI: 10.2210/pdb4jgf/pdb
• 分子名称: Gamma-crystallin D (2 entities in total)
• 機能のキーワード: structural protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 40809.41

構造登録者

Ji, F.L.,Koharudin, L.M.,Jung, J.W.,Gronenborn, A.M. (登録日: 2013-03-01, 公開日: 2013-05-22, 最終更新日: 2024-02-28)

主引用文献

Ji, F.,Koharudin, L.M.,Jung, J.,Gronenborn, A.M.
Crystal structure of the cataract-causing P23T gamma D-crystallin mutant.
Proteins, 81:1493-1498, 2013

PubMed Abstract: Up to now, efforts to crystallize the cataract-associated P23T mutant of human γD-crystallin have not been successful. Therefore, insights into the light scattering mechanism of this mutant have been exclusively obtained from solution work. Here we present the first crystal structure of the P23T mutant at 2.5 Å resolution. The protein exhibits essentially the same overall structure as seen for the wild-type protein. Based on our structural data, we confirm that no major conformational changes are caused by the mutation, and that solution phase properties of the mutant appear exclusively associated with cataract formation.

PubMed: 23670788
DOI: 10.1002/prot.24321

実験手法

X-RAY DIFFRACTION (2.5 Å)