4JF2

Structure of a class II preQ1 riboswitch reveals ligand recognition by a new fold

Summary for 4JF2

• Entry DOI: 10.2210/pdb4jf2/pdb
• Descriptor: PreQ1-II Riboswitch, 7-DEAZA-7-AMINOMETHYL-GUANINE, CESIUM ION, ... (5 entities in total)
• Functional Keywords: riboswitch, h-type pseudoknot, rna
• Biological source: Lactobacillus rhamnosus
• Total number of polymer chains: 1
• Total formula weight: 27120.56

Authors

Liberman, J.A.,Wedekind, J.E. (deposition date: 2013-02-27, release date: 2013-04-17, Last modification date: 2024-02-28)

Primary citation

Liberman, J.A.,Salim, M.,Krucinska, J.,Wedekind, J.E.
Structure of a class II preQ1 riboswitch reveals ligand recognition by a new fold.
Nat.Chem.Biol., 9:353-355, 2013

PubMed Abstract: PreQ1 riboswitches regulate genes by binding the pyrrolopyrimidine intermediate preQ1 during the biosynthesis of the essential tRNA base queuosine. We report what is to our knowledge the first preQ1-II riboswitch structure at 2.3-Å resolution, which uses a previously uncharacterized fold to achieve effector recognition at the confluence of a three-way helical junction flanking a pseudoknotted ribosome-binding site. The results account for translational control mediated by the preQ1-II riboswitch class and expand the known repertoire of ligand-binding modes used by regulatory RNAs.

PubMed: 23584677
DOI: 10.1038/nchembio.1231

Experimental method

X-RAY DIFFRACTION (2.28 Å)