4JEP

Crystal structure of Toxoplasma gondii nucleoside triphosphate diphosphohydrolase 1 (NTPDase1)

Summary for 4JEP

Entry DOI	10.2210/pdb4jep/pdb
Related	4A57 4A59 4A5A 4A5B
Descriptor	Nucleoside-triphosphatase 2 (1 entity in total)
Functional Keywords	hydrolase, phosphatase, ntpdase
Biological source	Toxoplasma gondii
Cellular location	Secreted: Q27895
Total number of polymer chains	2
Total formula weight	136152.42
Authors	Krug, U.,Totzauer, R.,Strater, N. (deposition date: 2013-02-27, release date: 2013-04-10, Last modification date: 2017-11-15)
Primary citation	Krug, U.,Totzauer, R.,Strater, N. The crystal structure of Toxoplasma gondii nucleoside triphosphate diphosphohydrolase 1 represents a conformational intermediate in the reductive activation mechanism of the tetrameric enzyme. Proteins, 81:1271-1276, 2013 Cited by PubMed Abstract: Toxoplasma gondii nucleoside triphosphate diphosphohydrolase (NTPDase) 1 was crystallized in an intermediate tetrameric conformation. The crystal structure is similar to that of T. gondii NTPDase3 and represents an inactive conformation as the activating disulfide bridge is not reduced and the active site cleft between the two domains of each monomer is open. However, the arrangement of the monomers within the tetramer differs from that of the inactive form of NTPDase3 and may represent an intermediate conformation on the path of the closure motion of the tetramer induced upon activation. PubMed: 23526564 DOI: 10.1002/prot.24288 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.1 Å)

Structure validation