4JEP

Crystal structure of Toxoplasma gondii nucleoside triphosphate diphosphohydrolase 1 (NTPDase1)

4JEP の概要

• エントリーDOI: 10.2210/pdb4jep/pdb
• 関連するPDBエントリー: 4A57 4A59 4A5A 4A5B
• 分子名称: Nucleoside-triphosphatase 2 (1 entity in total)
• 機能のキーワード: hydrolase, phosphatase, ntpdase
• 由来する生物種: Toxoplasma gondii
• 細胞内の位置: Secreted: Q27895
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 136152.42

構造登録者

Krug, U.,Totzauer, R.,Strater, N. (登録日: 2013-02-27, 公開日: 2013-04-10, 最終更新日: 2024-11-20)

主引用文献

Krug, U.,Totzauer, R.,Strater, N.
The crystal structure of Toxoplasma gondii nucleoside triphosphate diphosphohydrolase 1 represents a conformational intermediate in the reductive activation mechanism of the tetrameric enzyme.
Proteins, 81:1271-1276, 2013

PubMed Abstract: Toxoplasma gondii nucleoside triphosphate diphosphohydrolase (NTPDase) 1 was crystallized in an intermediate tetrameric conformation. The crystal structure is similar to that of T. gondii NTPDase3 and represents an inactive conformation as the activating disulfide bridge is not reduced and the active site cleft between the two domains of each monomer is open. However, the arrangement of the monomers within the tetramer differs from that of the inactive form of NTPDase3 and may represent an intermediate conformation on the path of the closure motion of the tetramer induced upon activation.

PubMed: 23526564
DOI: 10.1002/prot.24288

実験手法

X-RAY DIFFRACTION (3.1 Å)