4JCV

Crystal structure of the RecOR complex in an open conformation

4JCV の概要

• エントリーDOI: 10.2210/pdb4jcv/pdb
• 分子名称: Recombination protein RecR, DNA repair protein RecO, ZINC ION (3 entities in total)
• 機能のキーワード: homologous recombination, recfor pathway, dna binding, zinc-finger, dna damage, dna repair, recr, reco, recor, recombination
• 由来する生物種: Deinococcus radiodurans; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 162792.10

構造登録者

Radzimanowski, J.,McSweeney, S.,Timmins, J. (登録日: 2013-02-22, 公開日: 2013-08-07, 最終更新日: 2023-11-08)

主引用文献

Radzimanowski, J.,Dehez, F.,Round, A.,Bidon-Chanal, A.,McSweeney, S.,Timmins, J.
An 'open' structure of the RecOR complex supports ssDNA binding within the core of the complex.
Nucleic Acids Res., 41:7972-7986, 2013

PubMed Abstract: Efficient DNA repair is critical for cell survival and the maintenance of genome integrity. The homologous recombination pathway is responsible for the repair of DNA double-strand breaks within cells. Initiation of this pathway in bacteria can be carried out by either the RecBCD or the RecFOR proteins. An important regulatory player within the RecFOR pathway is the RecOR complex that facilitates RecA loading onto DNA. Here we report new data regarding the assembly of Deinococcus radiodurans RecOR and its interaction with DNA, providing novel mechanistic insight into the mode of action of RecOR in homologous recombination. We present a higher resolution crystal structure of RecOR in an 'open' conformation in which the tetrameric RecR ring flanked by two RecO molecules is accessible for DNA binding. We show using small-angle neutron scattering and mutagenesis studies that DNA binding does indeed occur within the RecR ring. Binding of single-stranded DNA occurs without any major conformational changes of the RecOR complex while structural rearrangements are observed on double-stranded DNA binding. Finally, our molecular dynamics simulations, supported by our biochemical data, provide a detailed picture of the DNA binding motif of RecOR and reveal that single-stranded DNA is sandwiched between the two facing oligonucleotide binding domains of RecO within the RecR ring.

PubMed: 23814185
DOI: 10.1093/nar/gkt572

実験手法

X-RAY DIFFRACTION (3.34 Å)