4JCJ

Crystal structure of Isl1 LIM domains with Ldb1 LIM-interaction domain

4JCJ の概要

• エントリーDOI: 10.2210/pdb4jcj/pdb
• 分子名称: Insulin gene enhancer protein ISL-1,LIM domain-binding protein 1, ZINC ION (2 entities in total)
• 機能のキーワード: zinc finger, transcriptional complex, zinc binding, nucleus, protein binding
• 由来する生物種: Mus musculus (Mouse); 詳細
• 細胞内の位置: Nucleus : P70662
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 58847.22

構造登録者

Gadd, M.S.,Jacques, D.A.,Guss, J.M.,Matthews, J.M. (登録日: 2013-02-21, 公開日: 2013-06-19, 最終更新日: 2024-10-30)

主引用文献

Gadd, M.S.,Jacques, D.A.,Nisevic, I.,Craig, V.J.,Kwan, A.H.,Guss, J.M.,Matthews, J.M.
A structural basis for the regulation of the LIM-homeodomain protein islet 1 (Isl1) by intra- and intermolecular interactions.
J.Biol.Chem., 288:21924-21935, 2013

PubMed Abstract: Islet 1 (Isl1) is a transcription factor of the LIM-homeodomain (LIM-HD) protein family and is essential for many developmental processes. LIM-HD proteins all contain two protein-interacting LIM domains, a DNA-binding homeodomain (HD), and a C-terminal region. In Isl1, the C-terminal region also contains the LIM homeobox 3 (Lhx3)-binding domain (LBD), which interacts with the LIM domains of Lhx3. The LIM domains of Isl1 have been implicated in inhibition of DNA binding potentially through an intramolecular interaction with or close to the HD. Here we investigate the LBD as a candidate intramolecular interaction domain. Competitive yeast-two hybrid experiments indicate that the LIM domains and LBD from Isl1 can interact with apparently low affinity, consistent with no detection of an intermolecular interaction in the same system. Nuclear magnetic resonance studies show that the interaction is specific, whereas substitution of the LBD with peptides of the same amino acid composition but different sequence is not specific. We solved the crystal structure of a similar but higher affinity complex between the LIM domains of Isl1 and the LIM interaction domain from the LIM-HD cofactor protein LIM domain-binding protein 1 (Ldb1) and used these coordinates to generate a homology model of the intramolecular interaction that indicates poorer complementarity for the weak intramolecular interaction. The intramolecular interaction in Isl1 may provide protection against aggregation, minimize unproductive DNA binding, and facilitate cofactor exchange within the cell.

PubMed: 23750000
DOI: 10.1074/jbc.M113.478586

実験手法

X-RAY DIFFRACTION (3 Å)