4JB9

Crystal structure of antibody VRC06 in complex with HIV-1 gp120 core

Summary for 4JB9

• Entry DOI: 10.2210/pdb4jb9/pdb
• Related: 4J6R
• Descriptor: clade A/E 93TH057 HIV-1 gp120 core, antibody VRC06 heavy chain, antibody VRC06 light chain, ... (5 entities in total)
• Functional Keywords: hiv-1, gp120, antibody, vrc06, glycoprotein, immune system, viral protein-immune system complex, viral protein/immune system
• Biological source: HUMAN IMMUNODEFICIENCY VIRUS 1; More
• Total number of polymer chains: 3
• Total formula weight: 89800.66

Authors

Kwon, Y.D.,Zhou, T.,Srivatsan, S.,Kwong, P.D. (deposition date: 2013-02-19, release date: 2013-05-08, Last modification date: 2024-12-25)

Primary citation

Georgiev, I.S.,Doria-Rose, N.A.,Zhou, T.,Kwon, Y.D.,Staupe, R.P.,Moquin, S.,Chuang, G.Y.,Louder, M.K.,Schmidt, S.D.,Altae-Tran, H.R.,Bailer, R.T.,McKee, K.,Nason, M.,O'Dell, S.,Ofek, G.,Pancera, M.,Srivatsan, S.,Shapiro, L.,Connors, M.,Migueles, S.A.,Morris, L.,Nishimura, Y.,Martin, M.A.,Mascola, J.R.,Kwong, P.D.
Delineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization.
Science, 340:751-756, 2013

PubMed Abstract: Serum characterization and antibody isolation are transforming our understanding of the humoral immune response to viral infection. Here, we show that epitope specificities of HIV-1-neutralizing antibodies in serum can be elucidated from the serum pattern of neutralization against a diverse panel of HIV-1 isolates. We determined "neutralization fingerprints" for 30 neutralizing antibodies on a panel of 34 diverse HIV-1 strains and showed that similarity in neutralization fingerprint correlated with similarity in epitope. We used these fingerprints to delineate specificities of polyclonal sera from 24 HIV-1-infected donors and a chimeric siman-human immunodeficiency virus-infected macaque. Delineated specificities matched published specificities and were further confirmed by antibody isolation for two sera. Patterns of virus-isolate neutralization can thus afford a detailed epitope-specific understanding of neutralizing-antibody responses to viral infection.

PubMed: 23661761
DOI: 10.1126/science.1233989

Experimental method

X-RAY DIFFRACTION (2.6 Å)