4J89

Different photochemical events of a genetically encoded aryl azide define and modulate GFP fluorescence

4J89 の概要

• エントリーDOI: 10.2210/pdb4j89/pdb
• 関連するPDBエントリー: 4J88 4J8A
• 分子名称: Green fluorescent protein, SULFATE ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: beta-barrel, fluorescent protein, chromophore by cyclisation, p-azido-l-phenylalanine, cytosol
• 由来する生物種: Aequorea victoria (Jellyfish)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57770.73

構造登録者

Reddington, S.C.,Jones, D.D.,Rizkallah, P.J.,Tippmann, E.M. (登録日: 2013-02-14, 公開日: 2013-06-26, 最終更新日: 2023-12-06)

主引用文献

Reddington, S.C.,Rizkallah, P.J.,Watson, P.D.,Pearson, R.,Tippmann, E.M.,Jones, D.D.
Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence.
Angew.Chem.Int.Ed.Engl., 52:5974-5977, 2013

PubMed Abstract: Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.

PubMed: 23620472
DOI: 10.1002/anie.201301490

実験手法

X-RAY DIFFRACTION (2.1 Å)