4J6W

Crystal structure of HFQ from Pseudomonas aeruginosa in complex with CTP

4J6W の概要

• エントリーDOI: 10.2210/pdb4j6w/pdb
• 関連するPDBエントリー: 1U1S 1U1T 3QUI 4J5Y 4J6X 4J6Y
• 分子名称: Protein hfq, CYTIDINE-5'-TRIPHOSPHATE, CYTIDINE-5'-MONOPHOSPHATE, ... (9 entities in total)
• 機能のキーワード: lsm, rna binding protein, rna chaperone, srna, mrna
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 59898.47

構造登録者

Nikulin, A.D.,Murina, V.,Lekontseva, N. (登録日: 2013-02-12, 公開日: 2013-07-31, 最終更新日: 2024-07-03)

主引用文献

Murina, V.,Lekontseva, N.,Nikulin, A.
Hfq binds ribonucleotides in three different RNA-binding sites.
Acta Crystallogr.,Sect.D, 69:1504-1513, 2013

PubMed Abstract: The Hfq protein forms a doughnut-shaped homohexamer that possesses RNA-binding activity. There are two distinct RNA-binding surfaces located on the proximal and the distal sides of the hexamer. The proximal side is involved in the binding of mRNA and small noncoding RNAs (sRNAs), while the distal side has an affinity for A-rich RNA sequences. In this work, the ability of various ribonucleotides to form complexes with Hfq from Pseudomonas aeruginosa has been tested using X-ray crystallography. ATP and ADPNP have been located in the distal R-site, which is a site for poly(A) RNA binding. UTP has been found in the so-called lateral RNA-binding site at the proximal surface. CTP has been found in both the distal R-site and the proximal U-binding site. GTP did not form a complex with Hfq under the conditions tested. The results have demonstrated the power of the crystallographic method for locating ribonucleotides and predicting single-stranded RNA-binding sites on the protein surface.

PubMed: 23897473
DOI: 10.1107/S090744491301010X

実験手法

X-RAY DIFFRACTION (1.8 Å)