4J2C

GARP-SNARE Interaction

4J2C の概要

• エントリーDOI: 10.2210/pdb4j2c/pdb
• 分子名称: Syntaxin-6, Vacuolar protein sorting-associated protein 51 homolog (3 entities in total)
• 機能のキーワード: tethering complex, protein transport
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Golgi apparatus membrane ; Single-pass type IV membrane protein : O43752; Golgi apparatus, trans-Golgi network : Q9UID3
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 28982.24

構造登録者

Abascal-Palacios, G.,Schindler, C.,Rojas, A.L.,Bonifacino, J.S.,Hierro, A. (登録日: 2013-02-04, 公開日: 2013-12-25, 最終更新日: 2024-02-28)

主引用文献

Abascal-Palacios, G.,Schindler, C.,Rojas, A.L.,Bonifacino, J.S.,Hierro, A.
Structural basis for the interaction of the Golgi-Associated Retrograde Protein Complex with the t-SNARE Syntaxin 6.
Structure, 21:1698-1706, 2013

PubMed Abstract: The Golgi-Associated Retrograde Protein (GARP) complex is a tethering factor involved in the fusion of endosome-derived transport vesicles to the trans-Golgi network through interaction with components of the Syntaxin 6/Syntaxin 16/Vti1a/VAMP4 SNARE complex. The mechanisms by which GARP and other tethering factors engage the SNARE fusion machinery are poorly understood. Herein, we report the structural basis for the interaction of the human Ang2 subunit of GARP with the Syntaxin 6 and the closely related Syntaxin 10. The crystal structure of the Syntaxin 6 Habc domain in complex with a peptide from the N terminus of Ang2 shows a binding mode in which a dityrosine motif of Ang2 interacts with a highly conserved groove in Syntaxin 6. Structure-based mutational analyses validate the crystal structure and support the phylogenetic conservation of this interaction.

PubMed: 23932592
DOI: 10.1016/j.str.2013.06.025

実験手法

X-RAY DIFFRACTION (1.801 Å)