4J19

Structure of a novel telomere repeat binding protein bound to DNA

4J19 の概要

• エントリーDOI: 10.2210/pdb4j19/pdb
• 分子名称: Homeobox-containing protein 1, DNA (5'-D(*CP*TP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*G)-3'), DNA (5'-D(*TP*CP*TP*AP*AP*CP*CP*CP*TP*AP*AP*CP*CP*CP*TP*AP*AP*CP*A)-3'), ... (5 entities in total)
• 機能のキーワード: telomere repeat binding, telomeric dna, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus . Isoform 5: Nucleus : Q6NT76
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 37865.87

構造登録者

Kappei, D.,Butter, F.,Benda, C.,Scheibe, M.,Draskovic, I.,Stevense, M.,Lopes Novo, C.,Basquin, C.,Krastev, D.B.,Kittler, R.,Jessberger, R.,Londono-Vallejo, A.J.,Mann, M.,Buchholz, F. (登録日: 2013-02-01, 公開日: 2013-05-29, 最終更新日: 2023-09-20)

主引用文献

Kappei, D.,Butter, F.,Benda, C.,Scheibe, M.,Draskovic, I.,Stevense, M.,Novo, C.L.,Basquin, C.,Araki, M.,Araki, K.,Krastev, D.B.,Kittler, R.,Jessberger, R.,Londono-Vallejo, J.A.,Mann, M.,Buchholz, F.
HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment.
Embo J., 32:1681-1701, 2013

PubMed Abstract: Telomeres are repetitive DNA structures that, together with the shelterin and the CST complex, protect the ends of chromosomes. Telomere shortening is mitigated in stem and cancer cells through the de novo addition of telomeric repeats by telomerase. Telomere elongation requires the delivery of the telomerase complex to telomeres through a not yet fully understood mechanism. Factors promoting telomerase-telomere interaction are expected to directly bind telomeres and physically interact with the telomerase complex. In search for such a factor we carried out a SILAC-based DNA-protein interaction screen and identified HMBOX1, hereafter referred to as homeobox telomere-binding protein 1 (HOT1). HOT1 directly and specifically binds double-stranded telomere repeats, with the in vivo association correlating with binding to actively processed telomeres. Depletion and overexpression experiments classify HOT1 as a positive regulator of telomere length. Furthermore, immunoprecipitation and cell fractionation analyses show that HOT1 associates with the active telomerase complex and promotes chromatin association of telomerase. Collectively, these findings suggest that HOT1 supports telomerase-dependent telomere elongation.

PubMed: 23685356
DOI: 10.1038/emboj.2013.105

実験手法

X-RAY DIFFRACTION (2.9 Å)