4J0M

Crystal structure of BRL1 (LRR) in complex with brassinolide

4J0M の概要

• エントリーDOI: 10.2210/pdb4j0m/pdb
• 分子名称: Serine/threonine-protein kinase BRI1-like 1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: leucine-rich repeat, receptor, transferase
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress, thale-cress)
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein: Q9ZWC8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 166232.67

構造登録者

Chai, J.,She, J.,Han, Z.,Zhou, B. (登録日: 2013-01-31, 公開日: 2013-07-24, 最終更新日: 2024-10-30)

主引用文献

She, J.,Han, Z.,Zhou, B.,Chai, J.
Structural basis for differential recognition of brassinolide by its receptors
Protein Cell, 4:475-482, 2013

PubMed Abstract: Brassinosteroids, a group of plant steroid hormones, regulate many aspects of plant growth and development. We and other have previously solved the crystal structures of BRI1(LRR) in complex with brassinolide, the most active brassinosteroid identified thus far. Although these studies provide a structural basis for the recognition of brassinolide by its receptor BRI1, it still remains poorly understood how the hormone differentiates among its conserved receptors. Here we present the crystal structure of the BRI1 homolog BRL1 in complex with brassinolide. The structure shows that subtle differences around the brassinolide binding site can generate a striking effect on its recognition by the BRI1 family of receptors. Structural comparison of BRL1 and BRI1 in their brassinolide-bound forms reveals the molecular basis for differential binding of brassinolide to its different receptors, which can be used for more efficient design of plant growth regulators for agricultural practice. On the basis of our structural studies and others' data, we also suggest possible mechanisms for the activation of BRI1 family receptors.

PubMed: 23709366
DOI: 10.1007/s13238-013-3027-8

実験手法

X-RAY DIFFRACTION (2.5 Å)