4IUD

Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in its as-isolated form with ascorbate - partly reduced state

Summary for 4IUD

• Entry DOI: 10.2210/pdb4iud/pdb
• Related: 4IUB 4IUC
• Descriptor: Uptake hydrogenase large subunit, Uptake hydrogenase small subunit, NI-FE OXIDIZED ACTIVE CENTER, ... (9 entities in total)
• Functional Keywords: [nife] hydrogenase, knallgas bacteria, proteobacteria, aerobic hydrogen bacteria, hydrogen catalysis, metalloenzyme, metalloprotein catalytic center, nickel-iron cofactor, bimetallic, ni-fe active site, iron-sulfur cluster, [4fe-3s] cluster, [3fe-4s] cluster, [4fe-4s] cluster, reduced state, oxidized state, oxygen-tolerant hydrogenase, membrane-bound, oxidoreductase
• Biological source: Ralstonia eutropha; More
• Cellular location: Cell membrane; Peripheral membrane protein: P31891 P31892
• Total number of polymer chains: 2
• Total formula weight: 105901.06

Authors

Hammer, M.,Schmidt, A.,Frielingsdorf, S.,Fritsch, J.,Lenz, O.,Scheerer, P. (deposition date: 2013-01-20, release date: 2014-04-02, Last modification date: 2023-12-06)

• Primary citation: Frielingsdorf, S.,Fritsch, J.,Schmidt, A.,Hammer, M.,Lowenstein, J.,Siebert, E.,Pelmenschikov, V.,Jaenicke, T.,Kalms, J.,Rippers, Y.,Lendzian, F.,Zebger, I.,Teutloff, C.,Kaupp, M.,Bittl, R.,Hildebrandt, P.,Friedrich, B.,Lenz, O.,Scheerer, P.; Reversible [4Fe-3S] cluster morphing in an O2-tolerant [NiFe] hydrogenase.; Nat.Chem.Biol., 10:378-385, 2014; PubMed: 24705592; DOI: 10.1038/nchembio.1500

Experimental method

X-RAY DIFFRACTION (1.45 Å)