4ISR

Binding domain of Botulinum neurotoxin DC in complex with rat synaptotagmin II

4ISR の概要

• エントリーDOI: 10.2210/pdb4isr/pdb
• 関連するPDBエントリー: 4ISQ
• 分子名称: Neurotoxin, Synaptotagmin-2, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: membrane binding, synaptotagmin and ganglioside binding, toxin
• 由来する生物種: Clostridium botulinum; 詳細
• 細胞内の位置: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass membrane protein: P29101
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 158917.37

構造登録者

Berntsson, R.P.-A.,Peng, L.,Svensson, L.M.,Dong, M.,Stenmark, P. (登録日: 2013-01-17, 公開日: 2013-08-14, 最終更新日: 2024-02-28)

主引用文献

Berntsson, R.P.,Peng, L.,Svensson, L.M.,Dong, M.,Stenmark, P.
Crystal Structures of Botulinum Neurotoxin DC in Complex with Its Protein Receptors Synaptotagmin I and II.
Structure, 21:1602-1611, 2013

PubMed Abstract: Botulinum neurotoxins (BoNTs) can cause paralysis at exceptionally low concentrations and include seven serotypes (BoNT/A-G). The chimeric BoNT/DC toxin has a receptor binding domain similar to the same region in BoNT/C. However, BoNT/DC does not share protein receptor with BoNT/C. Instead, it shares synaptotagmin (Syt) I and II as receptors with BoNT/B, despite their low sequence similarity. Here, we present the crystal structures of the binding domain of BoNT/DC in complex with the recognition domains of its protein receptors, Syt-I and Syt-II. The structures reveal that BoNT/DC possesses a Syt binding site, distinct from the established Syt-II binding site in BoNT/B. Structure-based mutagenesis further shows that hydrophobic interactions play a key role in Syt binding. The structures suggest that the BoNT/DC ganglioside binding sites are independent of the protein receptor binding site. Our results reveal the remarkable versatility in the receptor recognition of the BoNTs.

PubMed: 23932591
DOI: 10.1016/j.str.2013.06.026

実験手法

X-RAY DIFFRACTION (2.59 Å)