4IIJ

Crystal structure of tubulin-stathmin-TTL-apo complex

4IIJ の概要

• エントリーDOI: 10.2210/pdb4iij/pdb
• 関連するPDBエントリー: 4I4T 4I50 4I55 4IHJ
• 分子名称: Tubulin alpha-1B chain, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, Tubulin beta-2B chain, ... (11 entities in total)
• 機能のキーワード: alpha-tubulin, beta-tubulin, ligase, gtpase, microtubule, stathmin, cell cycle
• 由来する生物種: Rattus norvegicus (brown rat,rat,rats); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton: P81947 Q6B856; Golgi apparatus : P63043
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 264036.53

構造登録者

Prota, A.E.,Magiera, M.M.,Kuijpers, M.,Bargsten, K.,Frey, D.,Wieser, M.,Jaussi, R.,Hoogenraad, C.C.,Kammerer, R.A.,Janke, C.,Steinmetz, M.O. (登録日: 2012-12-20, 公開日: 2013-01-30, 最終更新日: 2023-09-20)

主引用文献

Prota, A.E.,Magiera, M.M.,Kuijpers, M.,Bargsten, K.,Frey, D.,Wieser, M.,Jaussi, R.,Hoogenraad, C.C.,Kammerer, R.A.,Janke, C.,Steinmetz, M.O.
Structural basis of tubulin tyrosination by tubulin tyrosine ligase.
J.Cell Biol., 200:259-270, 2013

PubMed Abstract: Tubulin tyrosine ligase (TTL) catalyzes the post-translational retyrosination of detyrosinated α-tubulin. Despite the indispensable role of TTL in cell and organism development, its molecular mechanism of action is poorly understood. By solving crystal structures of TTL in complex with tubulin, we here demonstrate that TTL binds to the α and β subunits of tubulin and recognizes the curved conformation of the dimer. Biochemical and cellular assays revealed that specific tubulin dimer recognition controls the activity of the enzyme, and as a consequence, neuronal development. The TTL-tubulin structure further illustrates how the enzyme binds the functionally crucial C-terminal tail sequence of α-tubulin and how this interaction catalyzes the tyrosination reaction. It also reveals how TTL discriminates between α- and β-tubulin, and between different post-translationally modified forms of α-tubulin. Together, our data suggest that TTL has specifically evolved to recognize and modify tubulin, thus highlighting a fundamental role of the evolutionary conserved tubulin tyrosination cycle in regulating the microtubule cytoskeleton.

PubMed: 23358242
DOI: 10.1083/jcb.201211017

実験手法

X-RAY DIFFRACTION (2.6 Å)