4IHX

Crystal structure of Fis bound to 27 bp 2-Aminopurine substituted DNA F28-2AP (AAATTTGTTTGA2T2TTGAGCAAATTT)

4IHX の概要

• エントリーDOI: 10.2210/pdb4ihx/pdb
• 関連するPDBエントリー: 4IHV 4IHW 4IHY
• 分子名称: DNA-binding protein fis, 27-bp DNA Strand A, 27-bp DNA Strand B (3 entities in total)
• 機能のキーワード: protein-dna complex, hth domain, minor groove compression, dna bending, indirect recognition, transcription-dna complex, transcription/dna
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 39090.65

構造登録者

Hancock, S.P.,Cascio, D.,Johnson, R.C. (登録日: 2012-12-19, 公開日: 2013-05-01, 最終更新日: 2023-09-20)

主引用文献

Hancock, S.P.,Ghane, T.,Cascio, D.,Rohs, R.,Di Felice, R.,Johnson, R.C.
Control of DNA minor groove width and Fis protein binding by the purine 2-amino group.
Nucleic Acids Res., 41:6750-6760, 2013

PubMed Abstract: The width of the DNA minor groove varies with sequence and can be a major determinant of DNA shape recognition by proteins. For example, the minor groove within the center of the Fis-DNA complex narrows to about half the mean minor groove width of canonical B-form DNA to fit onto the protein surface. G/C base pairs within this segment, which is not contacted by the Fis protein, reduce binding affinities up to 2000-fold over A/T-rich sequences. We show here through multiple X-ray structures and binding properties of Fis-DNA complexes containing base analogs that the 2-amino group on guanine is the primary molecular determinant controlling minor groove widths. Molecular dynamics simulations of free-DNA targets with canonical and modified bases further demonstrate that sequence-dependent narrowing of minor groove widths is modulated almost entirely by the presence of purine 2-amino groups. We also provide evidence that protein-mediated phosphate neutralization facilitates minor groove compression and is particularly important for binding to non-optimally shaped DNA duplexes.

PubMed: 23661683
DOI: 10.1093/nar/gkt357

実験手法

X-RAY DIFFRACTION (2.8 Å)