4IEG

Structure and interactions of the RNA-dependent RNA polymerase from bacteriophage phi12 (P1 crystal form)

Summary for 4IEG

• Entry DOI: 10.2210/pdb4ieg/pdb
• Related: 4GZK
• Descriptor: RNA-dependent RNA polymerase P2, MAGNESIUM ION (3 entities in total)
• Functional Keywords: rna-directed rna polymerase, transferase
• Biological source: Pseudomonas phage phi12
• Total number of polymer chains: 4
• Total formula weight: 301938.66

Authors

Ren, Z.,Franklin, M.C.,Ghose, R. (deposition date: 2012-12-13, release date: 2013-07-24, Last modification date: 2023-09-20)

Primary citation

Ren, Z.,C Franklin, M.,Ghose, R.
Structure of the RNA-directed RNA Polymerase from the cystovirus 12.
Proteins, 81:1479-1484, 2013

PubMed Abstract: We have determined the structure of P2, the self-priming RdRp from cystovirus φ12 in two crystal forms (A, B) at resolutions of 1.7 Å and 2.1 Å. Form A contains Mg(2+) bound at a site that deviates from the canonical noncatalytic position seen in form B. These structures provide insight into the temperature sensitivity of a ts-mutant. However, the tunnel through which template ssRNA accesses the active site is partially occluded by a flexible loop; this feature, along with suboptimal positioning of other structural elements that prevent the formation of a stable initiation complex, indicate an inactive conformation in crystallo.

PubMed: 23568335
DOI: 10.1002/prot.24297

Experimental method

X-RAY DIFFRACTION (2.1 Å)