4IDW
Polycrystalline T6 Bovine Insulin: Anisotropic Lattice Evolution and Novel Structure Refinement Strategy
Summary for 4IDW
| Entry DOI | 10.2210/pdb4idw/pdb |
| Descriptor | Insulin A chain, Insulin B chain, ZINC ION, ... (4 entities in total) |
| Functional Keywords | hormone, insulin family, carbohydrate metabolism, hormone-growth, t6 bovine insulin |
| Biological source | Bos taurus (Bovine) More |
| Cellular location | Secreted: P01317 P01317 |
| Total number of polymer chains | 4 |
| Total formula weight | 11617.96 |
| Authors | Margiolaki, I.,Giannopoulou, A.E.,Wright, J.P.,Knight, L.,Norrman, M.,Schluckebier, G.,Fitch, A.,Von Dreele, R.B. (deposition date: 2012-12-13, release date: 2013-06-05, Last modification date: 2024-10-30) |
| Primary citation | Margiolaki, I.,Giannopoulou, A.E.,Wright, J.P.,Knight, L.,Norrman, M.,Schluckebier, G.,Fitch, A.N.,Von Dreele, R.B. High-resolution powder X-ray data reveal the T6 hexameric form of bovine insulin Acta Crystallogr.,Sect.D, 69:978-990, 2013 Cited by PubMed Abstract: A series of bovine insulin samples were obtained as 14 polycrystalline precipitates at room temperature in the pH range 5.0-7.6. High-resolution powder X-ray diffraction data were collected to reveal the T6 hexameric insulin form. Sample homogeneity and reproducibility were verified by additional synchrotron measurements using an area detector. Pawley analyses of the powder patterns displayed pH- and radiation-induced anisotropic lattice modifications. The pronounced anisotropic lattice variations observed for T6 insulin were exploited in a 14-data-set Rietveld refinement to obtain an average crystal structure over the pH range investigated. Only the protein atoms of the known structure with PDB code 2a3g were employed in our starting model. A novel approach for refining protein structures using powder diffraction data is presented. In this approach, each amino acid is represented by a flexible rigid body (FRB). The FRB model requires a significantly smaller number of refinable parameters and restraints than a fully free-atom refinement. A total of 1542 stereochemical restraints were imposed in order to refine the positions of 800 protein atoms, two Zn atoms and 44 water molecules in the asymmetric unit using experimental data in the resolution range 18.2-2.7 Å for all profiles. PubMed: 23695242DOI: 10.1107/S0907444913003867 PDB entries with the same primary citation |
| Experimental method | POWDER DIFFRACTION |
Structure validation
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