4ICB

PROLINE CIS-TRANS ISOMERS IN CALBINDIN D9K OBSERVED BY X-RAY CRYSTALLOGRAPHY

4ICB の概要

• エントリーDOI: 10.2210/pdb4icb/pdb
• 分子名称: CALBINDIN D9K, CALCIUM ION (3 entities in total)
• 機能のキーワード: calcium-binding protein
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8721.91

構造登録者

Svensson, L.A. (登録日: 1991-08-27, 公開日: 1993-10-31, 最終更新日: 2024-02-28)

主引用文献

Svensson, L.A.,Thulin, E.,Forsen, S.
Proline cis-trans isomers in calbindin D9k observed by X-ray crystallography.
J.Mol.Biol., 223:601-606, 1992

PubMed Abstract: In a structure of recombinant bovine calbindin D9k, determined crystallographically to 1.6 A resolution, a proline in mixed, approximately equally populated, cis and trans conformation is observed. Isomers of this kind have not been reported in structure determinations of calbindin D9k to 2.3 A resolution or in any other crystallographically determined protein structure. The cis-trans isomerization occurs at the peptide bond between Gly42 and Pro43, which is in agreement with results from two-dimensional 1H nuclear magnetic resonance spectroscopy experiments on solutions of calbindin D9k. Alternative backbone stretches have been modeled and refined by stereochemical restrained least-squares refinement for the segment Lys41 to Pro43. The final R-value was 0.188. The structural perturbations accompanying the cis-trans isomerization are found to be very localized. The largest positional differences are observed at residue Gly42, in which the alternative positions of the oxygen atom are 3.6 A apart.

PubMed: 1542107
DOI: 10.1016/0022-2836(92)90976-Q

実験手法

X-RAY DIFFRACTION (1.6 Å)