4I9J

Structure of the N254Y/H258Y mutant of the phosphatidylinositol-specific phospholipase C from S. aureus bound to diC4PC

4I9J の概要

• エントリーDOI: 10.2210/pdb4i9j/pdb
• 関連するPDBエントリー: 4I8Y 4I90 4I9M 4I9T
• 分子名称: 1-phosphatidylinositol phosphodiesterase, ACETATE ION, (4S,7R)-7-(heptanoyloxy)-4-hydroxy-N,N,N-trimethyl-10-oxo-3,5,9-trioxa-4-phosphahexadecan-1-aminium 4-oxide, ... (4 entities in total)
• 機能のキーワード: tim barrel, phospholipase, dic4pc binding, lyase, hydrolase
• 由来する生物種: Staphylococcus aureus subsp. aureus
• 細胞内の位置: Secreted: P45723
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36822.99

構造登録者

Goldstein, R.I.,Cheng, J.,Stec, B.,Gershenson, A.,Roberts, M.F. (登録日: 2012-12-05, 公開日: 2013-04-10, 最終更新日: 2023-09-20)

主引用文献

Cheng, J.,Goldstein, R.,Gershenson, A.,Stec, B.,Roberts, M.F.
The cation-pi box is a specific phosphatidylcholine membrane targeting motif.
J.Biol.Chem., 288:14863-14873, 2013

PubMed Abstract: Peripheral membrane proteins can be targeted to specific organelles or the plasma membrane by differential recognition of phospholipid headgroups. Although molecular determinants of specificity for several headgroups, including phosphatidylserine and phosphoinositides are well defined, specific recognition of the headgroup of the zwitterionic phosphatidylcholine (PC) is less well understood. In cytosolic proteins the cation-π box provides a suitable receptor for choline recognition and binding through the trimethylammonium moiety. In PC, this moiety might provide a sufficient handle to bind to peripheral proteins via a cation-π cage, where the π systems of two or more aromatic residues are within 4-5 Å of the quaternary amine. We prove this hypothesis by engineering the cation-π box into secreted phosphatidylinositol-specific phospholipase C from Staphylococcus aureus, which lacks specific PC recognition. The N254Y/H258Y variant selectively binds PC-enriched vesicles, and x-ray crystallography reveals N254Y/H258Y binds choline and dibutyroylphosphatidylcholine within the cation-π motif. Such simple PC recognition motifs could be engineered into a wide variety of secondary structures providing a generally applicable method for specific recognition of PC.

PubMed: 23576432
DOI: 10.1074/jbc.M113.466532

実験手法

X-RAY DIFFRACTION (1.85 Å)