4I9F

Crystal structure of glycerol phosphate phosphatase Rv1692 from Mycobacterium tuberculosis in complex with calcium

4I9F の概要

• エントリーDOI: 10.2210/pdb4i9f/pdb
• 関連するPDBエントリー: 4I9G
• 分子名称: Glycerol 3-phosphate phosphatase, CALCIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: haloacid dehalogenase superfamily, phosphatase, glycerol 3-phosphate binding, hydrolase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 76315.58

構造登録者

Biswas, T.,Larrouy-Maumus, G.,de Carvalho, L.P.,Tsodikov, O.V. (登録日: 2012-12-05, 公開日: 2013-07-10, 最終更新日: 2023-09-20)

主引用文献

Larrouy-Maumus, G.,Biswas, T.,Hunt, D.M.,Kelly, G.,Tsodikov, O.V.,de Carvalho, L.P.
Discovery of a glycerol 3-phosphate phosphatase reveals glycerophospholipid polar head recycling in Mycobacterium tuberculosis.
Proc.Natl.Acad.Sci.USA, 110:11320-11325, 2013

PubMed Abstract: Functional assignment of enzymes encoded by the Mycobacterium tuberculosis genome is largely incomplete despite recent advances in genomics and bioinformatics. Here, we applied an activity-based metabolomic profiling method to assign function to a unique phosphatase, Rv1692. In contrast to its annotation as a nucleotide phosphatase, metabolomic profiling and kinetic characterization indicate that Rv1692 is a D,L-glycerol 3-phosphate phosphatase. Crystal structures of Rv1692 reveal a unique architecture, a fusion of a predicted haloacid dehalogenase fold with a previously unidentified GCN5-related N-acetyltransferase region. Although not directly involved in acetyl transfer, or regulation of enzymatic activity in vitro, this GCN5-related N-acetyltransferase region is critical for the solubility of the phosphatase. Structural and biochemical analysis shows that the active site features are adapted for recognition of small polyol phosphates, and not nucleotide substrates. Functional assignment and metabolomic studies of M. tuberculosis lacking rv1692 demonstrate that Rv1692 is the final enzyme involved in glycerophospholipid recycling/catabolism, a pathway not previously described in M. tuberculosis.

PubMed: 23801751
DOI: 10.1073/pnas.1221597110

実験手法

X-RAY DIFFRACTION (2.21 Å)